Hyaluronate Acid-Dependent Protection and Enhanced Corneal Wound Healing against Oxidative Damage in Corneal Epithelial Cells

Purpose. To evaluate the effects and mechanism of exogenous hyaluronate (HA) in promoting corneal wound healing. Methods. Human corneal epithelial cells (HCECs) were incubated with different concentrations of HA to evaluate their efficiency in promoting cell migration and their modulation of repair...

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Bibliographic Details
Published in:Journal of Ophthalmology 2016-01, Vol.2016 (2016), p.1-10-190
Main Authors: Yuan, Jin, Ling, Shiqi, Huang, Haixiang, Sun, Yifang, Wang, Bowen, Tian, Bishan, Deng, Yuqing, Zhong, Jing, Chen, Ling
Format: Article
Language:English
Subjects:
Analysis
Apoptosis
Cell adhesion & migration
Cell culture
Complications and side effects
Cornea
Cytokines
Defects
Dosage and administration
Epithelial cells
Extracellular matrix
Flow cytometry
Gene expression
Health aspects
Hyaluronic acid
Lubricants & lubrication
Physiological aspects
Wound healing
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Summary:Purpose. To evaluate the effects and mechanism of exogenous hyaluronate (HA) in promoting corneal wound healing. Methods. Human corneal epithelial cells (HCECs) were incubated with different concentrations of HA to evaluate their efficiency in promoting cell migration and their modulation of repair factors. After inducing hyperosmolar conditions, the cell morphologies, cell apoptosis, and expression levels of TNF-α and MMP-9 were detected to assess the protective role of HA. Corneal epithelium-injured rat models were established to test the therapeutic effects of 0.3% HA. Then, the wound healing rates, the RNA expression levels of inflammatory cytokines, and repair factors were examined. Results. HCECs in the 0.03% and 0.3% HA groups showed fewer morphological alterations and lower rates of cell apoptosis following preincubation with HA under hyperosmolar conditions, as well as the expression levels of MMP-9 and TNF-α. In the rat model, the areas of fluorescein staining in the corneas of 0.3% HA group were significantly smaller than the control group. The expression levels of IL-1β and MMP-9 were decreased, while CD44 and FN were increased in the 0.3% HA group. Conclusion. HA enhanced corneal epithelial cell wound healing by promoting cell migration, upregulating repair responses, and suppressing inflammatory responses.
ISSN:2090-004X
2090-0058
DOI:10.1155/2016/6538051