Integrated mRNA-seq and miRNA-seq analysis of goat fibroblasts response to Brucella Melitensis strain M5-90

Brucellosis is a globally zoonotic bacterial disease of humans and various animals including goats, sheep, and cattle. Brucella melitensis M5-90, a live attenuated vaccine strain, has been widely used to prevent brucellosis in goats and sheep. However, the molecular mechanisms governing protective i...

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Bibliographic Details
Published in:PeerJ (San Francisco, CA) CA), 2021-06, Vol.9, p.e11679-e11679, Article e11679
Main Authors: Li, Baobao, Chen, Si, Wang, Chengqiang, Chen, Qiaoling, Man, Churiga, An, Qi, Zhang, Zhenxing, Liu, Zhiyong, Du, Li, Wang, Fengyang
Format: Article
Language:English
Subjects:
Adaptive immunity
Analysis
Animals
Archives & records
Bacterial infections
Bioinformatics
Brucella melitensis strain M5-90
Brucellosis
Cell Biology
Cytotoxicity
Data analysis
Ethylenediaminetetraacetic acid
Fibroblasts
Gene expression
Goat fibroblasts
Goats
Health aspects
Immune response
Immunology
Immunomodulation
Infections
Infectious Diseases
Innate immunity
Interleukin 1
Interleukin 8
MAP kinase
Messenger RNA
MicroRNA
miRNA
Miscarriage
Molecular Biology
Molecular modelling
Natural killer cells
Pathogens
Phagocytes
Principal components analysis
Riboflavin
Signal transduction
Toll-like receptors
Transcriptomes
Transcriptomics
Tumor necrosis factor
Vaccines
Zoonoses
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Summary:Brucellosis is a globally zoonotic bacterial disease of humans and various animals including goats, sheep, and cattle. Brucella melitensis M5-90, a live attenuated vaccine strain, has been widely used to prevent brucellosis in goats and sheep. However, the molecular mechanisms governing protective immunity response in non-professional phagocytes infected with B. melitensis M5-90 have not been fully investigated, especially in goats. In our research, goat fibroblasts were used as in vitro models to determine these mechanisms by transcriptome analysis. After incubating with B. melitensis M5-90 3 h, the infected goat fibroblasts were collected at 0 h, 4 h, 24 h, 48 h and 72 h for RNA-seq. The results indicated that there were totally 11,819 differentially expressed genes (DEGs) and 777 differentially expressed (DE) miRNAs found in experiment groups compared with the control groups (|log2(Foldchange)|[greater than or equal to]1, FDR
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.11679