Molecular Detection of Southern Tomato Amalgavirus Prevalent in Tomatoes and Its Genomic Characterization with Global Evolutionary Dynamics

• Published in: Viruses 2022-11, Vol.14 (11), p.2481
• Main Authors: Hussain, Muhammad Dilshad, Farooq, Tahir, Chen, Xi, Jiang, Tong, Zang, Lianyi, Shakeel, Muhammad Taimoor, Zhou, Tao
• Format: Article
• Language: English
• Subjects: Amalgaviridae; Cloning; Coat protein; cryptic pathogen; Diseases and pests; DNA-directed RNA polymerase; Environmental aspects; Evolution; Genetic aspects; Genetic diversity; Genetic variability; Genomes; Genomics; Haplotypes; Infections; Natural history; Negative selection; Next-generation sequencing; Pakistan; Pathogens; phylodynamics; Phylogeny; Plant viruses; RNA; RNA Viruses - genetics; RNA-Dependent RNA Polymerase; Seeds; Solanum lycopersicum; southern tomato amalgavirus; Tomatoes; vertical transmission; viral prevalence; Virus diseases of plants; Viruses
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v14112481

Southern tomato amalgavirus (STV) is a cryptic pathogen that is abundant in tomato production fields and intensifies the resurgence of tomato yellow stunt disease (ToYSD), together with other phytoviruses. Here, we mapped the geographical and genomic diversity, phylogenetics, and evolutionary dynamics of STV. We found that STV prevailed across China and Pakistan, with a maximum average rate of infection of 43.19% in Beijing, China, and 40.08% in Punjab, Pakistan. Subsequently, we amplified, cloned, and annotated the complete genome sequences of STV isolates from L. in China (OP548653 and OP548652) and Pakistan (MT066231) using Sanger and next-generation sequencing (NGS). These STV isolates displayed close evolutionary relationships with others from Asia, America, and Europe. Whole-genome-based molecular diversity analysis showed that STV populations had 33 haplotypes with a gene diversity (Hd) of 0.977 and a nucleotide diversity (π) of 0.00404. The genetic variability of RNA-dependent RNA-polymerase (RdRp) was higher than that of the putative coat protein (CP) p42. Further analysis revealed that STV isolates were likely to be recombinant but with a lower-to-moderate level of confidence. With a variable distribution pattern of positively and negatively selected sites, negative selection pressure predominantly acted on p42 and RdRp. These findings elaborated on the molecular variability and evolutionary trends among STV populations across major tomato-producing regions of the world.