Changes in Trophic Groups of Protists With Conversion of Rainforest Into Rubber and Oil Palm Plantations

Protists, abundant but enigmatic single-celled eukaryotes, are important soil microbiota providing numerous ecosystem functions. We employed high-throughput sequencing of environmental DNA, targeting the V4 region of the 18S rRNA gene, to characterize changes in their abundance, species richness, an...

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Bibliographic Details
Published in:Frontiers in microbiology 2019-02, Vol.10, p.240-240
Main Authors: Schulz, Garvin, Schneider, Dominik, Brinkmann, Nicole, Edy, Nur, Daniel, Rolf, Polle, Andrea, Scheu, Stefan, Krashevska, Valentyna
Format: Article
Language:English
Subjects:
community composition
high-throughput sequencing
Indonesia
Microbiology
rainforest transformation
soil protist diversity
V4 region of 18S rRNA
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Summary:Protists, abundant but enigmatic single-celled eukaryotes, are important soil microbiota providing numerous ecosystem functions. We employed high-throughput sequencing of environmental DNA, targeting the V4 region of the 18S rRNA gene, to characterize changes in their abundance, species richness, and community structure with conversion of lowland rainforest into rubber agroforest (jungle rubber), and rubber and oil palm plantations; typical agricultural systems in Sumatra, Indonesia. We identified 5,204 operational taxonomic units (OTUs) at 97% identity threshold of protists from 32 sites. Protists species richness was similar in rainforest, jungle rubber and oil palm plantations but significantly lower in rubber plantations. After standardization, 4,219 OTUs were assigned to five trophic groups, and inspected for effects of land-use change, and potential biotic and abiotic driving factors. The most abundant trophic group was phagotrophs (52%), followed by animal parasites (29%), photoautotrophs (12%), plant parasites (1%), and symbionts (
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2019.00240