A Study on How Methionine Restriction Decreases the Body's Hepatic and Lipid Deposition in Rice Field Eel ( Monopterus albus )

• Published in: International journal of molecular sciences 2021-12, Vol.22 (24), p.13379
• Main Authors: Hu, Yajun, Cai, Minglang, Zhong, Huan, Chu, Wuying, Hu, Yi
• Format: Article
• Language: English
• Subjects: Acetyl-CoA Carboxylase - metabolism; Amino acids; Animals; Apolipoproteins; Body fat; Carnitine; China; Cholesterol; Deposition; Diet; Dietary Supplements; Esterification; Fatty acids; Fatty Acids - metabolism; Fatty liver; Fatty Liver - metabolism; Gene expression; hepatic lipid metabolism; hepatic structure; High density lipoprotein; Lipase; Lipid metabolism; Lipid Metabolism - drug effects; Lipid Metabolism - physiology; Lipids; Lipids - physiology; Lipoproteins, VLDL - metabolism; Liver - metabolism; Metabolic disorders; Methionine; Methionine - deficiency; Methionine - metabolism; Methionine - pharmacology; Monopterus albus; Nutrition research; Protein expression; Protein synthesis; Proteins; RNA, Messenger - metabolism; Smegmamorpha - metabolism; Soybeans; Sterol Esterase - metabolism; Triglycerides; Triglycerides - metabolism
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms222413379

Methionine restriction reduces animal lipid deposition. However, the molecular mechanism underlying how the body reacts to the condition and regulates lipid metabolism remains unknown. In this study, a feeding trial was performed on rice field eel with six isonitrogenous and isoenergetic feeds that included different levels of methionine (0, 2, 4, 6, 8, and 10 g/kg). Compared with M0 (0 g/kg), the crude lipid and crude protein of increased markedly in M8 (8 g/kg) ( < 0.05), serum (total cholesterol, triglyceride, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and non-esterified free fatty acids), and hepatic contents (hepatic lipase, apolipoprotein-A, fatty acid synthetase, total cholesterol, triglyceride, and lipoprteinlipase). However, in the serum, very-low-density lipoprotein and hepatic contents (hormone-sensitive triglyceride lipase, Acetyl CoA carboxylase, carnitine palmitoyltransterase, and mirosomal triglygeride transfer protein) decreased markedly in M8 ( < 0.05). The contents of hepatic C18:2n-6, C22:6n-3, and n-3PUFA in the M8 group were significantly higher than those in M0 ( < 0.05), and the contents of lipid droplets in M8 were higher than those in M0. Compared with M0, the hepatic gcn2, eif2α , and were remarkably downregulated in M8, while and were markedly upregulated in M8. Moreover, hepatic SREBP1 and FAS protein expression were upregulated significantly in M8 ( < 0.01). In short, methionine restriction decreased the lipid deposition of , especially for hepatic lipid deposition, and mainly downregulated hepatic fatty acid metabolism. Besides, could be activated under methionine restriction.