Transport of Ca2+ and Ca2+-Dependent Permeability Transition in the Liver and Heart Mitochondria of Rats with Different Tolerance to Acute Hypoxia

The work examines the kinetic parameters of Ca2+ uptake via the mitochondrial calcium uniporter complex (MCUC) and the opening of the Ca2+-dependent permeability transition pore (MPT pore) in the liver and heart mitochondria of rats with high resistance (HR) and low resistance (LR) to acute hypoxia....

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Bibliographic Details
Published in:Biomolecules (Basel, Switzerland) Switzerland), 2020-01, Vol.10 (1), p.114
Main Authors: Belosludtsev, Konstantin, Dubinin, Mikhail, Talanov, Eugeny, Starinets, Vlada, Tenkov, Kirill, Zakharova, Nadezhda, Belosludtseva, Natalia
Format: Article
Language:English
Subjects:
ATP synthase
Calcium (mitochondrial)
Calcium homeostasis
Calcium influx
Calcium permeability
Calcium transport
cyclophilin d
Enzymes
Heart
Homeostasis
Hypoxia
Laboratory animals
Liver
Membrane permeability
Metabolism
Mitochondria
mitochondrial ca2+-induced permeability transition pore
mitochondrial calcium transport
mitochondrial calcium uniporter complex
Mitochondrial permeability transition pore
Musculoskeletal system
Permeability
Phenotypes
Proteins
resistance to hypoxia
Statistical analysis
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Summary:The work examines the kinetic parameters of Ca2+ uptake via the mitochondrial calcium uniporter complex (MCUC) and the opening of the Ca2+-dependent permeability transition pore (MPT pore) in the liver and heart mitochondria of rats with high resistance (HR) and low resistance (LR) to acute hypoxia. We found that the rate of Ca2+ uptake by mitochondria of the liver and heart in HR rats is higher than that in LR rats, which is associated with a higher level of the channel-forming subunit MCU in liver mitochondria of HR rats and a lower content of the dominant-negative channel subunit MCUb in heart mitochondria of HR rats. It was shown that the liver mitochondria of HR rats are more resistant to the induction of the MPT pore than those of LR rats (the calcium retention capacity of liver mitochondria of HR rats was found to be 1.3 times greater than that of LR rats). These data correlate with the fact that the level of F0F1-ATP synthase, a possible structural element of the MPT pore, in the liver mitochondria of HR rats is lower than in LR rats. In heart mitochondria of rats of the two phenotypes, no statistically significant difference in the formation of the MPT pore was revealed. The paper discusses how changes in the expression of the MCUC subunits and the putative components of the MPT pore can affect Ca2+ homeostasis of mitochondria in animals with originally different tolerance to hypoxia and in hypoxia-induced tissue injury.
ISSN:2218-273X
2218-273X
DOI:10.3390/biom10010114