Development of a Multiplex Bead Assay for Simultaneous Serodiagnosis of Antibodies against Mycobacterium bovis , Brucella suis , and Trichinella spiralis in Wild Boar

The aim of this study was to evaluate the diagnostic performance of a multiplex bead assay for the simultaneous detection of antibodies against , , and . Sera from Eurasian wild boar of known serological status for TB (64 seropositive, 106 seronegative), (30 seropositive, 39 seronegative), and (21 s...

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Published in:Microorganisms (Basel) 2021-04, Vol.9 (5), p.904
Main Authors: Touloudi, Antonia, Valiakos, George, Cawthraw, Shaun, Kostoulas, Polychronis, Gortázar, Christian, Boadella, Mariana, Giannakopoulos, Alexios, Birtsas, Periklis, Sofia, Marina, Athanasiou, Labrini V, Satra, Maria, Athanasakopoulou, Zoi, Kantere, Maria, Spyrou, Vassiliki, Petrovska, Liljana, Billinis, Charalambos
Format: Article
Language:English
Subjects:
Aluminum
Animals
Antibodies
Antigens
Assaying
Beads
Brucella suis
Diagnostic systems
Hogs
MPB83 antigen
multiplex bead assay
Multiplexing
Mycobacterium bovis
Pathogens
Performance evaluation
serological assay
Serology
Sus scrofa
Trichinella spiralis
Tuberculosis
wild boar
Zoonoses
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Summary:The aim of this study was to evaluate the diagnostic performance of a multiplex bead assay for the simultaneous detection of antibodies against , , and . Sera from Eurasian wild boar of known serological status for TB (64 seropositive, 106 seronegative), (30 seropositive, 39 seronegative), and (21 seropositive, 97 seronegative) were used for the development and evaluation of the assay. Magnetic beads coated with recombinant MPB83 antigen (TB), a whole-cell 1330 antigen, and an E/S antigen were used for the detection of specific antibodies using Bio-Rad Bio-Plex technology. The sensitivities (Se) and specificities (Sp) of the multiplex assay were, for , 0.98 and 0.86; for , 1.00 and 0.97; and for , 0.90 and 0.99 (Se and Sp, respectively). The results show the diagnostic potential of this assay for the simultaneous detection of antibodies against , , and in wild boar.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms9050904