Rapid detection of high-risk HPV16 and HPV18 based on microchip electrophoresis

Researches on detection of human papillomavirus (HPV) high-risk samples were carried out by polymerase chain reaction (PCR) coupled with microchip electrophoresis (MCE). Herein, we introduced a simple, rapid, automated method for detecting high-risk samples HPV16 and HPV18. In this research, general...

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Bibliographic Details
Published in:Journal of pharmaceutical analysis 2020-08, Vol.10 (4), p.329-333
Main Authors: Fan, Zhaoxuan, Feng, Xiao, Zhang, Weifei, Zhang, Xueji, Lin, Jin-Ming
Format: Article
Language:English
Subjects:
Automation
Biotechnology
Cervical cancer
Cervix
Deoxyribonucleic acid
Detection
DNA
DNA analysis
Human papillomavirus
Infections
Medical screening
Microchip electrophoresis
Original
Polymerase chain reaction
Semiconductors
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Summary:Researches on detection of human papillomavirus (HPV) high-risk samples were carried out by polymerase chain reaction (PCR) coupled with microchip electrophoresis (MCE). Herein, we introduced a simple, rapid, automated method for detecting high-risk samples HPV16 and HPV18. In this research, general primers were initially selected to obtain sufficient detectable yield by PCR to verify feasibility of MCM method for HPV detection, then type-specific primers were further used to evaluate the specificity of MCE method. The results indicated MCE method was capable of specifically detecting high-risk HPV16 and HPV18, and also enabled simultaneous detection of multiplex samples. This MCE method described here has been successfully applied to HPV detection and displayed excellent reliability demonstrating by sequencing results. The inherent capability of MCE facilitated HPV detection conducted in a small chip with automated, high throughput, massive parallelized analysis. We envision that MCE method will definitely pave a way for clinical diagnosis, and even on-site screening of cervical cancer. This work described a rapid, automatic assay based on MCE for detecting high risk samples of HPV16 and HPV18. [Display omitted] •A rapid, automatic assay was established for HPV detection.•The described method exhibited the advantages of facile operation mode.•The proposed method is of high sensitivity, specificity and reproducibility.
ISSN:2095-1779
2214-0883
DOI:10.1016/j.jpha.2020.04.003