Cloning and characterization of a pyrethroid pesticide decomposing esterase gene, Est3385, from Rhodopseudomonas palustris PSB-S

• Published in: Scientific reports 2018-05, Vol.8 (1), p.7384-8, Article 7384
• Main Authors: Luo, Xiangwen, Zhang, Deyong, Zhou, Xuguo, Du, Jiao, Zhang, Songbai, Liu, Yong
• Format: Article
• Language: English
• Subjects: 13/1; 38/23; 38/77; 631/61/168; 704/172; 82/29; 82/80; 82/83; Agricultural ecosystems; Amino acid sequence; Amino acids; Cloning; Detoxification; E coli; Enzymatic activity; Esterase; Genomes; Humanities and Social Sciences; Metal ions; multidisciplinary; Nucleotide sequence; Pesticide residues; Pesticides; Phylogeny; Protein engineering; Pyrethroids; Rhodopseudomonas palustris; Science; Science (multidisciplinary); Substrate specificity
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-25734-9

Full length open reading frame of pyrethroid detoxification gene, Est3385 , contains 963 nucleotides. This gene was identified and cloned based on the genome sequence of Rhodopseudomonas palustris PSB-S available at the GneBank. The predicted amino acid sequence of Est3385 shared moderate identities (30–46%) with the known homologous esterases. Phylogenetic analysis revealed that Est3385 was a member in the esterase family I. Recombinant Est3385 was heterologous expressed in E. coli , purified and characterized for its substrate specificity, kinetics and stability under various conditions. The optimal temperature and pH for Est3385 were 35 °C and 6.0, respectively. This enzyme could detoxify various pyrethroid pesticides and degrade the optimal substrate fenpropathrin with a K m and V max value of 0.734 ± 0.013 mmol·l −1 and 0.918 ± 0.025 U·µg −1 , respectively. No cofactor was found to affect Est3385 activity but substantial reduction of enzymatic activity was observed when metal ions were applied. Taken together, a new pyrethroid degradation esterase was identified and characterized. Modification of Est3385 with protein engineering toolsets should enhance its potential for field application to reduce the pesticide residue from agroecosystems.