Nephrotoxicity of polymyxin B: experimental study in cells and implications for nursing practice

The aim of the study was to characterize the cell damage mechanisms involved in the pathophysiology of cytotoxicity of polymyxin B in proximal tubular cells (LLC - PK1) and discuss about the nurses interventions to identify at risk patients and consider prevention or treatment of nephrotoxicity acut...

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Published in:Revista da Escola de Enfermagem da U S P 2014-04, Vol.48 (2), p.272-277
Main Authors: Neiva, Luciana Barros de Moura, Borges, Fernanda Teixeira, Watanabe, Mirian, Pessoa, Edson de Andrade, Barbosa, Dulce Aparecida, Vattimo, Maria de Fatima Fernandes
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - adverse effects
Antibacterianos
Atención de enfermería
Células LLC-PK1
Kidney Diseases - chemically induced
Kidney Diseases - nursing
Kidney Diseases - prevention & control
Lesión renal aguda
LLC-PK1 Cells
NURSING
Polimixina B
Polymyxin B - adverse effects
Swine
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Summary:The aim of the study was to characterize the cell damage mechanisms involved in the pathophysiology of cytotoxicity of polymyxin B in proximal tubular cells (LLC - PK1) and discuss about the nurses interventions to identify at risk patients and consider prevention or treatment of nephrotoxicity acute kidney injury. This is a quantitative experimental in vitro study, in which the cells were exposed to 375μM polymyxin B sulfate concentration. Cell viability was determined by exclusion of fluorescent dyes and morphological method with visualization of apoptotic bodies for fluorescence microscopy. Cells exposed to polymyxin B showed reduced viability, increased number of apoptotic cells and a higher concentration of the enzyme lactate dehydrogenase. The administration of polymyxin B in vitro showed the need for actions to minimize adverse effects such as nephrotoxicity.
ISSN:0080-6234
1980-220X
0080-6234
DOI:10.1590/S0080-6234201400002000011