Hypoxic enhancement of exosome release by breast cancer cells

Exosomes are nanovesicles secreted by tumour cells which have roles in paracrine signalling during tumour progression, including tumour-stromal interactions, activation of proliferative pathways and bestowing immunosuppression. Hypoxia is an important feature of solid tumours which promotes tumour p...

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Bibliographic Details
Published in:BMC cancer 2012-09, Vol.12 (1), p.421-421, Article 421
Main Authors: King, Hamish W, Michael, Michael Z, Gleadle, Jonathan M
Format: Article
Language:English
Subjects:
Angiogenesis
Breast cancer
Breast cancer cells
Breast Neoplasms - genetics
Breast Neoplasms - metabolism
Cancer cells
Cell culture
Cell Fractionation - methods
Cell growth
Cell Hypoxia
Cell Line, Tumor
Cell Proliferation
Cell Survival
Culture Media, Conditioned - chemistry
Development and progression
DNA repair
ExoquickTM
Exosomes
Exosomes - genetics
Exosomes - metabolism
Female
Gene Expression Regulation, Neoplastic
Genetic aspects
Genetic transcription
Hepatology
Humans
Hypoxia
Hypoxia-Inducible Factor 1 - metabolism
Immunotherapy
Life sciences
Medical research
Metastasis
MicroRNAs - genetics
MicroRNAs - metabolism
Nanoparticle tracking analysis
Nanoparticles
Nanosight
Physiological aspects
Proteins
Studies
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Summary:Exosomes are nanovesicles secreted by tumour cells which have roles in paracrine signalling during tumour progression, including tumour-stromal interactions, activation of proliferative pathways and bestowing immunosuppression. Hypoxia is an important feature of solid tumours which promotes tumour progression, angiogenesis and metastasis, potentially through exosome-mediated signalling. Breast cancer cell lines were cultured under either moderate (1% O2) or severe (0.1% O2) hypoxia. Exosomes were isolated from conditioned media and quantitated by nanoparticle tracking analysis (NTA) and immunoblotting for the exosomal protein CD63 in order to assess the impact of hypoxia on exosome release. Hypoxic exosome fractions were assayed for miR-210 by real-time reverse transcription polymerase chain reaction and normalised to exogenous and endogenous control genes. Statistical significance was determined using the Student T test with a P value of 
ISSN:1471-2407
1471-2407
DOI:10.1186/1471-2407-12-421