Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms

Depending on the environmental conditions, cells adapt their metabolism and specific growth rate. Rearrangements occur on many different levels such as macromolecular composition, gene and protein expression, morphology and metabolic flux patterns. As the interplay of these processes also determines...

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Bibliographic Details
Published in:Microorganisms (Basel) 2018-06, Vol.6 (3), p.56
Main Authors: Peebo, Karl, Neubauer, Peter
Format: Article
Language:English
Subjects:
chemostat
continuous cultures
continuous manufacturing
recombinant protein production
Review
specific growth rate
steady state
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Summary:Depending on the environmental conditions, cells adapt their metabolism and specific growth rate. Rearrangements occur on many different levels such as macromolecular composition, gene and protein expression, morphology and metabolic flux patterns. As the interplay of these processes also determines the output of a recombinant protein producing system, having control over specific growth rate of the culture is advantageous. Continuous culture methods were developed to grow cells in a constant environment and have been used for decades to study basic microbial physiology in a controlled and reproducible manner. Our review summarizes the uses of continuous cultures in cell physiology studies and process development, with a focus on recombinant protein-producing microorganisms.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms6030056