Leucine-Rich Glioma-Inactivated 1 (LGI1) Protein Stimulates Proliferation and IL-10 Production in Peripheral Blood Mononuclear Cells of Patients with LGI1 Antibody-Mediated Autoimmune Encephalitis In Vitro

• Published in: International journal of molecular sciences 2024-02, Vol.25 (5), p.2581
• Main Authors: Goihl, Alexander, Reinhold, Dirk, Reinhold, Annegret, Schraven, Burkhart, Körtvelyessy, Peter
• Format: Article
• Language: English
• Subjects: Antibodies; Antigens; Autoimmune diseases; Autoimmune Diseases of the Nervous System; autoimmune encephalitis; B cells; Cell culture; Cell differentiation; Communication; Convulsions & seizures; cytokine production; Cytokines; Dendritic cells; Disease; Encephalitis; Glioma; Humans; IL-10; Immunoglobulin G; Immunology; Interleukin-10; Intracellular Signaling Peptides and Proteins; Kinases; Leucine; Leukocytes, Mononuclear - pathology; LGI1 antibody-mediated LE; Limbic Encephalitis; Lymphocytes; Patients; Proteins; Seizures (Medicine); T cell proliferation; T cells
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25052581

Limbic encephalitis (LE) due to anti-leucine-rich glioma-inactivated 1 (LGI1) antibodies is an autoimmune disease characterized by distinct clinical features unique to LGI1 LE, such as faciobrachial dystonic seizures. However, it is unclear whether an additional disease-related LGI1 antigen-specific T cell response is involved in the pathogenesis of this disease. To address this question, we studied the effect of recombinant LGI1 on the proliferation and effector-specific cytokine production (IFN-γ, IL-5, IL-10, and IL-17) of peripheral blood mononuclear cells (PBMCs) from patients with LGI1 LE and healthy controls. We observed that recombinant LGI1 stimulated the proliferation of PBMCs from patients with LGI1 LE, but not from healthy controls. Cytokine measurement of cell culture supernatants from PBMCs incubated with recombinant LGI1 revealed a highly significant increase in IL-10 release in PBMCs from patients with LGI1 LE in comparison with healthy controls. These results suggest that LGI1-mediated stimulation of PBMCs from patients with LGI1 LE leads to the establishment of an IL-10-dominated immunosuppressive cytokine milieu, which may inhibit Th1 differentiation and support B cell proliferation, IgG production, and IgG subclass switching.