The Interaction between Trolox and 4,4'-diisothiocyanatostilbene-2,2'-disulfonic Acid on Hypoxic Pulmonary Vasoconstriction in the Isolated Rabbit Lung

The mechanism of hypoxic pulmonary vasoconstriction (HPV) is still debatable. It has been proposed that reactive oxygen species (ROS) might be involved in HPV. However, there is no special transporter for superoxide anion in the cell membrane and it may release from the cells via anion exchanger. Th...

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Bibliographic Details
Published in:Iranian journal of medical sciences 2017-05, Vol.42 (3), p.284-291
Main Authors: Mansoori, Somayh, Moosavi, Seyed Mostafa Shid, Ketabchi, Farzaneh
Format: Article
Language:English
Subjects:
4.4’-diisothiocyanostilbene-2.2’-disulfonic acid
6-hydroxy-2.5.7.8-tetramethylchroman-2-carboxylic acid
Health aspects
Hypoxia
Ion transport
Lung
Lungs
Metabolites
Original
Properties
Pulmonary arteries
Reactive oxygen species
Vasoconstriction
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Summary:The mechanism of hypoxic pulmonary vasoconstriction (HPV) is still debatable. It has been proposed that reactive oxygen species (ROS) might be involved in HPV. However, there is no special transporter for superoxide anion in the cell membrane and it may release from the cells via anion exchanger. Therefore, the aim of this study was to investigate the interaction of ROS and anion exchanger in acute HPV. The present study was performed in the isolated rabbit lung. After preparation, the lungs were divided into four hypoxic groups of control, Trolox (antioxidant)-treated, 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS, anion exchanger inhibitor)-treated, and Trolox+DIDS-treated. Pulmonary artery pressure, left atrial pressure, and lung weight were continuously registered and PVR was then calculated. PO , PCO , HCO , pH, and NO metabolites of the perfusate were measured during steady-state and at the end of experiments (30 minutes). All data were compared with ANOVA and -test and significance was considered when P
ISSN:0253-0716
1735-3688