Human NANOS1 Represses Apoptosis by Downregulating Pro-Apoptotic Genes in the Male Germ Cell Line

• Published in: International journal of molecular sciences 2020-04, Vol.21 (8), p.3009
• Main Authors: Janecki, Damian M, Ilaslan, Erkut, Smialek, Maciej J, Sajek, Marcin P, Kotecki, Maciej, Ginter-Matuszewska, Barbara, Krainski, Patryk, Jaruzelska, Jadwiga, Kusz-Zamelczyk, Kamila
• Format: Article
• Language: English
• Subjects: Alleles; Amino Acid Substitution; Apoptosis; Apoptosis - genetics; Apoptosis Regulatory Proteins - genetics; Cell cycle; Cell Cycle - genetics; Cell growth; Cell Line; Cell Proliferation; Colorimetry; Communication; Flow cytometry; Gene expression; Gene Expression Regulation; Genes; Germ cells; Germ Cells - metabolism; human infertility; Humans; Infertility; Infertility - genetics; Insects; Maintenance; Male; Mutation; NANOS1; NANOS1 mutation; Polymerase chain reaction; Proteins; Reverse transcription; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Sequence analysis; Transfection; Tubules
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21083009

While two mouse NANOS paralogues, NANOS2 and NANOS3, are crucial for maintenance of germ cells by suppression of apoptosis, the mouse NANOS1 paralogue does not seem to regulate these processes. Previously, we described a human NANOS1 p.[(Pro34Thr);(Ser83del)] mutation associated with the absence of germ cells in seminiferous tubules of infertile patients, which might suggest an anti-apoptotic role of human NANOS1. In this study, we aimed to determine a potential influence of human NANOS1 on the maintenance of TCam-2 model germ cells by investigating proliferation, cell cycle, and apoptosis. Constructs encoding wild-type or mutated human NANOS1 were used for transfection of TCam-2 cells, in order to investigate the effect of NANOS1 on cell proliferation, which was studied using a colorimetric assay, as well as apoptosis and the cell cycle, which were measured by flow cytometry. RNA-Seq (RNA sequencing) analysis followed by RT-qPCR (reverse transcription and quantitative polymerase chain reaction) was conducted for identifying pro-apoptotic genes repressed by NANOS1. Here, we show that overexpression of NANOS1 downregulates apoptosis in TCam-2 cells. Moreover, we found that NANOS1 represses a set of pro-apoptotic genes at the mRNA level. We also found that the infertility-associated p.[(Pro34Thr);(Ser83del)] mutation causes NANOS1 to functionally switch from being anti-apoptotic to pro-apoptotic in the human male germ cell line. Thus, this report is the first to show an anti-apoptotic role of NANOS1 exerted by negative regulation of mRNAs of pro-apoptotic genes.