The relationships among Leishmania infantum and phyllostomid bats assessed by histopathological and molecular assays

• Published in: International journal for parasitology. Parasites and wildlife 2024-04, Vol.23, p.100904-100904, Article 100904
• Main Authors: da Silva, Alanderson Rodrigues, Herrera, Heitor Miraglia, de Oliveira, Carina Elisei, Torres, Jaire Marinho, Ferreira, Ana Maria Reis, Leite, Juliana da Silva, Menezes, Rodrigo Caldas, Martinez, Érica Verneque, de Oliveira, Gabrielly Moreira dos Santos, Santos, Filipe Martins, de Andrade, Gisele Braziliano
• Format: Article
• Language: English
• Subjects: Chiropteran; Dead-end host; Histopathology; Immunohistochemistry; Leishmaniasis
• ISSN: 2213-2244; 2213-2244
• DOI: 10.1016/j.ijppaw.2023.100904

Bats have been reported as reservoir host of Leishmania spp. worldwide, mostly by molecular detection. However, it is still unclear whether bats act as reservoirs of Leishmania infantum to sandflies vectors. In this sense, the investigation of amastigotes forms in the target organs, and the characterization of their associated inflammation, may help to clarify the epidemiological importance of bats in endemic areas for leishmaniasis. The aim of this work was to investigate the host-parasite relationships under microscopic evaluation and predict the epidemiological role of two phyllostomid bats species naturally infected by L. infantum in an endemic area for human leishmaniasis. Fragments of skin, liver and spleen of L. infantum positive and negative bats (Artibeus planirostris and Carollia perspicillata) by qPCR, were studied by histological and immunohistochemical techniques. Both groups, positive and negative, did not show differences in the histopathological study, presenting only discrete tissue changes. Liver and skin showed mild inflammatory reactions. Findings on spleen consisted of reactivity of the lymphoid follicles, expressive presence of apoptotic cells and macrophages containing abundant phagocytic cells debris. We did not find amastigote forms in tissues by histological and IHC techniques in positive qPCR bats. Our results allow us to hypothesize that phyllostomid bats seem to have an important role in reducing the risk of transmission, possibly acting as dead-end host. [Display omitted] •Relationship between phyllostomid bats and Leishmania infantum at microenvironment.•Amastigote forms were not detected in the skin, liver and spleen tissues of phyllostomid bats positive for L. infantum by PCR.•Phyllostomid bats may act as dead-end hosts for L. infantum.