A novel PCR-based point-of-care method enables rapid, sensitive and reliable diagnosis of Babesia gibsoni infection in dogs

Babesia gibsoni (B. gibsoni) is an intraerythrocytic protozoan parasite of dogs that causes fever and hemolytic illness. A timely diagnosis is essential for the disease management. Here, we report a QubeMDx PCR system which enables a rapid, sensitive and reliable diagnosis of B. gibsoni near the dog...

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Bibliographic Details
Published in:BMC veterinary research 2019-11, Vol.15 (1), p.428-428, Article 428
Main Authors: Liu, I-Li, Chi, Nai-Yu, Chang, Chia-Ling, Hung, Ming-Long, Chiu, Chun-Ta, Chen, Hui-Wen
Format: Article
Language:English
Subjects:
Analysis
Animals
Babesia - isolation & purification
Babesia gibsoni
Babesiosis - blood
Babesiosis - diagnosis
Babesiosis - parasitology
Deoxyribonucleic acid
Diagnosis
DNA
Dog Diseases - blood
Dog Diseases - diagnosis
Dog Diseases - parasitology
Dogs
EDTA
Fever
Health aspects
Infections
Methodology
Methods
Novels
Parasitemia
Parasites
Point-of-care
Point-of-Care Systems
Protozoa
QubeMDx PCR system
Real-time PCR
Real-Time Polymerase Chain Reaction - methods
Real-Time Polymerase Chain Reaction - veterinary
Sensitivity and Specificity
Validity
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Summary:Babesia gibsoni (B. gibsoni) is an intraerythrocytic protozoan parasite of dogs that causes fever and hemolytic illness. A timely diagnosis is essential for the disease management. Here, we report a QubeMDx PCR system which enables a rapid, sensitive and reliable diagnosis of B. gibsoni near the dog patient. Within 30 min, this diagnostic assay was able to detect as low as 0.002% parasitemia of the dog blood. Using clinical samples, this new assay was validated to demonstrate 100% agreement with real-time PCR. This novel diagnostic method provides a reliable point-of-care test to assist in the identification of B. gibsoni.
ISSN:1746-6148
1746-6148
DOI:10.1186/s12917-019-2181-5