Advances in Chemical and Biological Methods to Identify Microorganisms-From Past to Present

Fast detection and identification of microorganisms is a challenging and significant feature from industry to medicine. Standard approaches are known to be very time-consuming and labor-intensive (e.g., culture media and biochemical tests). Conversely, screening techniques demand a quick and low-cos...

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Bibliographic Details
Published in:Microorganisms (Basel) 2019-05, Vol.7 (5), p.130
Main Authors: Franco-Duarte, Ricardo, Černáková, Lucia, Kadam, Snehal, Kaushik, Karishma S, Salehi, Bahare, Bevilacqua, Antonio, Corbo, Maria Rosaria, Antolak, Hubert, Dybka-Stępień, Katarzyna, Leszczewicz, Martyna, Relison Tintino, Saulo, Alexandrino de Souza, Veruska Cintia, Sharifi-Rad, Javad, Coutinho, Henrique Douglas Melo, Martins, Natália, Rodrigues, Célia F
Format: Article
Language:English
Subjects:
Biochemical tests
chromogenic media
Classification
Cost analysis
Culture media
DNA fingerprinting
Food
Gene sequencing
Genetic engineering
Genotype & phenotype
Identification
Identification methods
MALDI-TOF
Metabolism
Microorganisms
microscopy techniques
Molecular biology
Organisms
Pathogens
PCR
Polymerase chain reaction
RAPD PCR
real-time PCR
Review
rRNA 16S
Taxonomy
WGS
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Summary:Fast detection and identification of microorganisms is a challenging and significant feature from industry to medicine. Standard approaches are known to be very time-consuming and labor-intensive (e.g., culture media and biochemical tests). Conversely, screening techniques demand a quick and low-cost grouping of bacterial/fungal isolates and current analysis call for broad reports of microorganisms, involving the application of molecular techniques (e.g., 16S ribosomal RNA gene sequencing based on polymerase chain reaction). The goal of this review is to present the past and the present methods of detection and identification of microorganisms, and to discuss their advantages and their limitations.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms7050130