Phytochemical Analysis of Agrimonia pilosa Ledeb, Its Antioxidant Activity and Aldose Reductase Inhibitory Potential

• Published in: International journal of molecular sciences 2017-02, Vol.18 (2), p.379-379
• Main Authors: Kim, Set Byeol, Hwang, Seung Hwan, Suh, Hong-Won, Lim, Soon Sung
• Format: Article
• Language: English
• Subjects: 1,1-diphenyl-2-picrylhydrazyl (DPPH); Agrimonia; Agrimonia - chemistry; Agrimonia pilosa Ledeb; Aldehyde reductase; Aldehyde Reductase - antagonists & inhibitors; aldose reductase; Antioxidants - chemistry; Antioxidants - pharmacology; Butanol; Catechin; Chromatography, High Pressure Liquid; Diabetes mellitus; diabetic complication; Enzyme Activation; Flavonoids; Flavonoids - chemistry; Free Radical Scavengers - chemistry; Free Radical Scavengers - pharmacology; Free radicals; Glucosides; Kinetics; Molecular Structure; Phytochemicals - chemistry; Plant Extracts - chemistry; Plant Extracts - pharmacology; Rodents; Sorbitol
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms18020379

The aim of this study was to determine aldose reductase (AR) inhibitory activity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity of compounds from Ledeb (AP). We isolated agrimoniin (AM), four flavonoid glucosides and two flavonoid glucuronides from the -butanol fraction of AP 50% methanol extract. In addition to isolated compounds, the AR-inhibitory activity and the DPPH free radical scavenging activity of catechin, 5-flavonoids, and 4-flavonoid glucosides (known components of AP) against rat lens AR (RLAR) and DPPH assay were measured. AM showed IC values of 1.6 and 13.0 μM against RLAR and DPPH scavenging activity, respectively. Additionally, AM, luteolin-7- -glucuronide (LGN), quercitrin (QU), luteolin (LT) and afzelin (AZ) showed high inhibitory activity against AR and were first observed to decrease sorbitol accumulation in the rat lens under high-sorbitol conditions ex vivo with inhibitory values of 47.6%, 91.8%, 76.9%, 91.8% and 93.2%, respectively. Inhibition of recombinant human AR by AM, LGN and AZ exhibited a noncompetitive inhibition pattern. Based on our results, AP and its constituents may play partial roles in RLAR and oxidative radical inhibition. Our results suggest that AM, LGN, QU, LT and AZ may potentially be used as natural drugs for treating diabetic complications.