HPTLC determination of diosgenin in fenugreek seeds

A new HPTLC-densitometric method for diosgenin determination in fenugreek seeds was established after optimization of the conditions for efficient saponin extraction and acid hydrolysis. Several procedures were tested, the best of which was a three-step Soxhlet extraction, followed by hydrolysis of...

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Bibliographic Details
Published in:Acta Pharmaceutica 2018-03, Vol.68 (1), p.97-107
Main Authors: Król-Kogus, Barbara, Lamine, Khenifi Mohammed, Migas, Piotr, Boudjeniba, Messaoud, Krauze-Baranowska, Mirosława
Format: Article
Language:English
Subjects:
Acetates - chemistry
Acetic acid
Chromatography, High Pressure Liquid - methods
Densitometers
diosgenin
Diosgenin - chemistry
Ethyl acetate
Fenugreek
fenugreek (Trigonella foenum-graecum)
Heptanes
HPTLC
Hydrolysis
Optimization
Plant Extracts - chemistry
Quantitative analysis
Reagents
Saponins
Saponins - chemistry
Seeds
Seeds - chemistry
Spraying
Sulfuric acid
Trigonella - chemistry
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Summary:A new HPTLC-densitometric method for diosgenin determination in fenugreek seeds was established after optimization of the conditions for efficient saponin extraction and acid hydrolysis. Several procedures were tested, the best of which was a three-step Soxhlet extraction, followed by hydrolysis of the obtained methanolic extract with 2 mol L H SO . Best diosgenin separation from other hydrolysis products was obtained on HPTLC Si60 plates u sing a mixture of n-heptane/ethyl acetate (7:3, V/V) and modified anisaldehyde as a spraying reagent. The method was preliminarily validated and the determined amounts of diosgenin in fenugreek seeds of Polish and African origin were found to be similar and ranged from 0.12-0.18 %.
ISSN:1846-9558
1330-0075
1846-9558
DOI:10.2478/acph-2018-0002