Detection of chromosome instability by interphase FISH in mouse and human tissues

• Published in: STAR protocols 2021-09, Vol.2 (3), p.100631-100631, Article 100631
• Main Authors: Torres-Ruiz, Raul, Grazioso, Tatiana P., Brandt, Marta, Martinez-Lage, Marta, Rodriguez-Perales, Sandra, Djouder, Nabil
• Format: Article
• Language: English
• Subjects: Cell Biology; In Situ Hybridization; Microscopy; Molecular Biology; Protocol; Single Cell
• ISSN: 2666-1667; 2666-1667
• DOI: 10.1016/j.xpro.2021.100631

Chromosomal instability (CIN), a type of genomic instability, favors changes in chromosome number and structure and it is associated with the progression and initiation of multiple diseases, including cancer. Therefore, CIN identification and analysis represents a useful tool for cancer diagnosis and treatment. Here, we report an optimized molecular cytogenetic protocol to detect CIN in formalin-fixed, paraffin-embedded mouse and human tissues, using fluorescent in situ hybridization to visualize and quantify chromosomal alterations such as amplifications, deletions, and translocations. For complete information on the generation and use of this protocol, please refer to Brandt et al. (2018). [Display omitted] •A protocol for single-cell CIN analysis in paraffin-embedded tissues•Design of FISH probes for CIN detection•Identification, visualization, and quantification of CIN by FISH Chromosomal instability (CIN), a type of genomic instability, favors changes in chromosome number and structure and it is associated with the progression and initiation of multiple diseases, including cancer. Therefore, CIN identification and analysis represents a useful tool for cancer diagnosis and treatment. Here, we report an optimized molecular-cytogenetic protocol to detect CIN in formalin-fixed, paraffin-embedded mouse and human tissues, using fluorescent in situ hybridization to visualize and quantify chromosomal alterations such as amplifications, deletions, and translocations.