Identification of Internal Reference Genes in Peripheral Blood Mononuclear Cells of Cattle Populations Adapted to Hot Arid Normoxia and Cold Arid Hypoxia Environments

To estimate gene expression in a reliable manner, quantitative real-time polymerase chain reaction data require normalisation using a panel of stably expressed reference genes (RGs). To date, information on an appropriate panel of RGs in cattle populations reared at cold arid high-altitude hypoxia a...

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Bibliographic Details
Published in:Frontiers in genetics 2022-02, Vol.12, p.730599-730599
Main Authors: Verma, Preeti, Sharma, Ankita, Sodhi, Monika, Tiwari, Manish, Vivek, Prince, Kataria, Ranjit S, Nirajan, S K, Bharti, Vijay K, Singh, Pawan, Lathwal, S S, Sharma, Vishal, Masharing, Nampher, Mukesh, Manishi
Format: Article
Language:English
Subjects:
cold arid
expression stability
Genetics
hot arid
normalization
qRT-PCR
reference genes
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Summary:To estimate gene expression in a reliable manner, quantitative real-time polymerase chain reaction data require normalisation using a panel of stably expressed reference genes (RGs). To date, information on an appropriate panel of RGs in cattle populations reared at cold arid high-altitude hypoxia and hot arid tropical normoxia environments is not available. Therefore, the present study was carried out to identify a panel of stably expressed RGs from 10 candidate genes ( and ) in peripheral blood mononuclear cells (PBMCs) of cattle populations reared at cold arid high-altitude hypoxia and hot arid normoxia environments. Four different statistical algorithms: geNorm, NormFinder, BestKeeper, and RefFinder were used to assess the stability of these genes. A total of 30 blood samples were collected: six adult heifers each of Ladakhi (LAC) and Holstein Frisian crosses (HFX) and 4 Jersey (JYC) cows from cold arid high-altitude hypoxia environments (group I) and five adult heifers each of Sahiwal (SAC), Karan Fries (KFC), and Holstein Friesian (HFC) cows from hot arid normoxia environments (group II). Combined analysis of group I and group II resulted in identification of a panel of RGs like and that could act as a useful resource to unravel the accurate transcriptional profile of PBMCs from diverse cattle populations adapted to distinct altitudes.
ISSN:1664-8021
1664-8021
DOI:10.3389/fgene.2021.730599