Assessment of Tie2-Rejuvenated Nucleus Pulposus Cell Transplants from Young and Old Patient Sources Demonstrates That Age Still Matters

• Published in: International journal of molecular sciences 2024-08, Vol.25 (15), p.8335
• Main Authors: Otani, Yuto, Schol, Jordy, Sakai, Daisuke, Nakamura, Yoshihiko, Sako, Kosuke, Warita, Takayuki, Tamagawa, Shota, Ambrosio, Luca, Munesada, Daiki, Ogasawara, Shota, Matsushita, Erika, Kawachi, Asami, Naiki, Mitsuru, Sato, Masato, Watanabe, Masahiko
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Age; Age Factors; Aged; Analysis; Animals; Backache; Cell Differentiation; Cell Proliferation; cell therapy; Cells; Cells, Cultured; Degenerative disc disease; disc degeneration; Female; Flow cytometry; Humans; intervertebral disc; Intervertebral Disc - cytology; Intervertebral Disc - metabolism; Intervertebral Disc Degeneration - therapy; Male; Middle Aged; Nucleus Pulposus - cytology; Nucleus Pulposus - metabolism; progenitor cells; Receptor, TIE-2 - genetics; Receptor, TIE-2 - metabolism; Regeneration; Stem Cell Transplantation - methods; Stem Cells - cytology; Stem Cells - metabolism; Tie2; Transplants & implants; Young Adult
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25158335

Cell transplantation is being actively explored as a regenerative therapy for discogenic back pain. This study explored the regenerative potential of Tie2 nucleus pulposus progenitor cells (NPPCs) from intervertebral disc (IVD) tissues derived from young (60 years of age) patient donors. We employed an optimized culture method to maintain Tie2 expression in NP cells from both donor categories. Our study revealed similar Tie2 positivity rates regardless of donor types following cell culture. Nevertheless, clear differences were also found, such as the emergence of significantly higher (3.6-fold) GD2 positivity and reduced (2.7-fold) proliferation potential for older donors compared to young sources. Our results suggest that, despite obtaining a high fraction of Tie2 NP cells, cells from older donors were already committed to a more mature phenotype. These disparities translated into functional differences, influencing colony formation, extracellular matrix production, and in vivo regenerative potential. This study underscores the importance of considering age-related factors in NPPC-based therapies for disc degeneration. Further investigation into the genetic and epigenetic alterations of Tie2 NP cells from older donors is crucial for refining regenerative strategies. These findings shed light on Tie2 NPPCs as a promising cell source for IVD regeneration while emphasizing the need for comprehensive understanding and scalability considerations in culture methods for broader clinical applicability.