Neuronal expression in Drosophila of an evolutionarily conserved metallophosphodiesterase reveals pleiotropic roles in longevity and odorant response

• Published in: PLoS genetics 2023-09, Vol.19 (9), p.e1010962-e1010962
• Main Authors: Gupta, Kriti, Chakrabarti, Sveta, Janardan, Vishnu, Gogia, Nishita, Banerjee, Sanghita, Srinivas, Swarna, Mahishi, Deepthi, Visweswariah, Sandhya S
• Format: Article
• Language: English
• Subjects: Amino acids; Analysis; Animals; Antennal lobe; Antimicrobial Peptides; Biology and Life Sciences; Brain; Cyclic adenylic acid; Cyclic AMP; Cyclic GMP; Desiccation; Drosophila; Drosophila - metabolism; Drosophila melanogaster - genetics; Drosophila melanogaster - metabolism; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Enzymes; Evolution; Females; Gene expression; Genes; Genetic aspects; Genetic engineering; Identification and classification; Insects; Life span; Longevity; Longevity - genetics; Mammals - metabolism; Methods; Neurons; Odorants; Organisms; Ovaries; Physical Sciences; Pleiotropy; Proteins; Research and Analysis Methods; RNA; RNA sequencing
• ISSN: 1553-7404; 1553-7390; 1553-7404
• DOI: 10.1371/journal.pgen.1010962

Evolutionarily conserved genes often play critical roles in organismal physiology. Here, we describe multiple roles of a previously uncharacterized Class III metallophosphodiesterase in Drosophila, an ortholog of the MPPED1 and MPPED2 proteins expressed in the mammalian brain. dMpped, the product of CG16717, hydrolyzed phosphodiester substrates including cAMP and cGMP in a metal-dependent manner. dMpped is expressed during development and in the adult fly. RNA-seq analysis of dMppedKO flies revealed misregulation of innate immune pathways. dMppedKO flies showed a reduced lifespan, which could be restored in Dredd hypomorphs, indicating that excessive production of antimicrobial peptides contributed to reduced longevity. Elevated levels of cAMP and cGMP in the brain of dMppedKO flies was restored on neuronal expression of dMpped, with a concomitant reduction in levels of antimicrobial peptides and restoration of normal life span. We observed that dMpped is expressed in the antennal lobe in the fly brain. dMppedKO flies showed defective specific attractant perception and desiccation sensitivity, correlated with the overexpression of Obp28 and Obp59 in knock-out flies. Importantly, neuronal expression of mammalian MPPED2 restored lifespan in dMppedKO flies. This is the first description of the pleiotropic roles of an evolutionarily conserved metallophosphodiesterase that may moonlight in diverse signaling pathways in an organism.