Transcriptomic Analysis Revealed Reactive Oxygen Species Scavenging Mechanisms Associated With Ferrous Iron Toxicity in Aromatic Keteki Joha Rice

Lowland acidic soils with water-logged regions are often affected by ferrous iron (Fe ) toxicity, a major yield-limiting factor of rice production. Under severe Fe toxicity, reactive oxygen species (ROS) are crucial, although molecular mechanisms and associated ROS homeostasis genes are still unknow...

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Bibliographic Details
Published in:Frontiers in plant science 2022-02, Vol.13, p.798580-798580
Main Authors: Regon, Preetom, Dey, Sangita, Rehman, Mehzabin, Pradhan, Amit Kumar, Chowra, Umakanta, Tanti, Bhaben, Talukdar, Anupam Das, Panda, Sanjib Kumar
Format: Article
Language:English
Subjects:
Fe homeostasis
Fe2+ toxicity
Plant Science
RNA-Seq
ROS
transcriptome
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Summary:Lowland acidic soils with water-logged regions are often affected by ferrous iron (Fe ) toxicity, a major yield-limiting factor of rice production. Under severe Fe toxicity, reactive oxygen species (ROS) are crucial, although molecular mechanisms and associated ROS homeostasis genes are still unknown. In this study, a comparative RNA-Seq based transcriptome analysis was conducted to understand the Fe toxicity tolerance mechanism in aromatic Keteki Joha. About 69 Fe homeostasis related genes and their homologs were identified, where most of the genes were downregulated. Under severe Fe toxicity, the biosynthesis of amino acids, RNA degradation, and glutathione metabolism were induced, whereas phenylpropanoid biosynthesis, photosynthesis, and fatty acid elongation were inhibited. The , and were upregulated in different tissues, suggesting the importance of Fe retention and sequestration for detoxification. However, several antioxidants, ROS scavenging genes and abiotic stress-responsive transcription factors indicate ROS homeostasis as one of the most important defense mechanisms under severe Fe toxicity. Catalase (CAT), glutathione (GSH), ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), and glutathione reductase (GR) were upregulated. Moreover, abiotic stress-responsive transcription factors, no apical meristem (NAC), myeloblastosis (MYB), auxin response factor (ARF), basic helix-loop-helix (bZIP), WRKY, and C2H2-zinc finger protein (C2H2-ZFP) were also upregulated. Accordingly, ROS homeostasis has been proposed as an essential defense mechanism under such conditions. Thus, the current study may enrich the understanding of Fe-homeostasis in rice.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2022.798580