The Use of Mesenchymal Stem Cells in the Complex Treatment of Kidney Tuberculosis (Experimental Study)

• Published in: Biomedicines 2022-11, Vol.10 (12), p.3062
• Main Authors: Muraviov, Alexander N, Vinogradova, Tatiana I, Remezova, Anna N, Ariel, Boris M, Gorelova, Anna A, Orlova, Nadezhda V, Yudintceva, Natalia M, Esmedliaeva, Diljara S, Dyakova, Marina E, Dogonadze, Marine Z, Zabolotnykh, Natalia V, Garapach, Irina A, Maslak, Olga S, Kirillov, Yuri A, Timofeev, Sergei E, Krylova, Yulia S, Yablonskiy, Petr K
• Format: Article
• Language: English
• Subjects: Abdomen; Bone marrow; Cell therapy; Ceruloplasmin; Connective tissues; Cytokines; Diabetes; Elastase; Epidemiology; experimental nephrotuberculosis; Health aspects; Infections; Inflammation; Kidneys; Laboratory animals; Mesenchymal stem cells; MSC; Nanoparticles; Parenchyma; Performance evaluation; Proteinase; Proteinase inhibitors; Rabbits; Renal failure; reparative reaction; Sclerosis; Stem cell transplantation; Stem cells; Tissue engineering; Tomography; Tuberculosis; Veins & arteries
• ISSN: 2227-9059; 2227-9059
• DOI: 10.3390/biomedicines10123062

In recent years, the application of mesenchymal stem cells (MSCs) has been recognized as a promising method for treatment of different diseases associated with inflammation and sclerosis, which include nephrotuberculosis. The aim of our study is to investigate the effectiveness of MSCs in the complex therapy of experimental rabbit kidney tuberculosis and to evaluate the effect of cell therapy on the reparative processes. Methods: To simulate kidney tuberculosis, a suspension of the standard strain Mycobacterium tuberculosis H37Rv (10 CFU) was used, which was injected into the cortical layer of the lower pole parenchyma of the left kidney under ultrasound control in rabbits. Anti-tuberculosis therapy (aTBT) was started on the 18th day after infection. MSCs (5 × 10 cells) were transplanted intravenously after the start of aTBT. Results: 2.5 months after infection, all animals showed renal failure. Conducted aTBT significantly reduced the level of albumin, ceruloplasmin, elastase and the severity of disorders in the proteinase/inhibitor system and increased the productive nature of inflammation. A month after MSC transplantation, the level of inflammatory reaction activity proteins decreased, the area of specific and destructive inflammation in kidneys decreased and the formation of mature connective tissue was noted, which indicates the reparative reaction activation.