HPLC-based metabolomics of Dendrobium officinale revealing its antioxidant ability

is an orchid with medicinal and nutritional properties that has received increasing attention because of its health benefits; however, there is limited information about the metabolic basis of these properties. In this report, secondary metabolites and the antioxidant activity of stem samples from t...

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Bibliographic Details
Published in:Frontiers in plant science 2023-01, Vol.14, p.1060242-1060242
Main Authors: Luo, Zhengfei, Liu, Lian, Nie, Qiong, Huang, Mingjin, Luo, Chunlii, Sun, Yedong, Ma, Yongyan, Yu, Jianxin, Du, Fuqiang
Format: Article
Language:English
Subjects:
antioxidant activity
Dendrobium officinale
differential metabolites
flavonoid
metabolic profiling
Plant Science
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Summary:is an orchid with medicinal and nutritional properties that has received increasing attention because of its health benefits; however, there is limited information about the metabolic basis of these properties. In this report, secondary metabolites and the antioxidant activity of stem samples from three provenances were analyzed, using a UHPLC-QqQ-MS/MS-based metabolomics approach. In total, 411 metabolites were identified including 8 categories such as flavonoids and phenolic acids, 136 of which were differential metabolites. These differentially accumulated metabolites (DAMs) were mainly enriched in secondary metabolic pathways such as flavone, flavonol, tropane, piperidine, pyridine, isoquinoline alkaloid biosynthesis and tyrosine metabolism. The metabolomic profiling suggested that the quantity and content of flavonoid compounds accounted for the highest proportion of total metabolites. Hierarchical cluster analysis (HCA) showed that the marker metabolites of from the three provenances were mainly flavonoids, alkaloids and phenolic acids. Correlation analysis identified that 48 differential metabolites showed a significant positive correlation with antioxidant capacity (r ³ 0.8 and p < 0.0092), and flavonoids were the main factors affecting the different antioxidant activities. It is worth noting that quercetin-3-O-sophoroside-7-O-rhamnoside and dihydropinosylvin methyl ether might be the main compounds causing the differences in antioxidant capacity of Yunnan provenance (YN), Zhejiang provenance (ZJ), and Guizhou provenance (GZ). These finding provides valuable information for screening varieties, quality control and product development of .
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2023.1060242