Genome-wide analysis of Hsp40 and Hsp70 gene family in four cotton species provides insights into their involvement in response to Verticillium dahliae and abiotic stress

• Published in: Frontiers in genetics 2023-01, Vol.14, p.1120861-1120861
• Main Authors: Zhou, Xin, Su, Ling, Tang, Rui, Dong, Yuxuan, Wang, Fei, Li, Rong, Xie, Quanliang, Zhang, Xianliang, Xiao, Guanghui, Li, Hongbin
• Format: Article
• Language: English
• Subjects: abiotic stress response; cotton; Genetics; HSP40 gene family; HSP70 gene family; Verticillium wilt resistance
• ISSN: 1664-8021; 1664-8021
• DOI: 10.3389/fgene.2023.1120861

Cotton is an important economic crop to provide natural fibers as raw materials to textile industry, and is significantly affected by biotic and abiotic stress during the whole growth stage, in which wilt (VW) caused by is one of the most destructive disease to lead to a significant yield reduction. Heat shock proteins (Hsps) are important molecular chaperones, and play crucial roles in plant growth, development, resistance to biotic and abiotic stress. Hsp40 and Hsp70 are two key Hsps in cell chaperone network, however, the function and regulatory mechanism of Hsp40 and Hsp70 members in VW resistance and abiotic stress in cotton are largely unknown. Herein, a systematic and comprehensive analysis of Hsp40s and Hsp70s in four cotton species of , , , and were performed. A total of 291 Hsp40s and 171 Hsp70s identified in four Gossypium species. Sequence analysis revealed that all Hsp40 proteins contained J domain that provides the binding sites to Hsp70. Protein-protein interaction prediction analysis displayed that GhHsp40-55 might interact with GhHsp70-2 and GhHsp70-13, suggesting their potential function as protein complex. Promoter cis-acting element analysis demonstrated that multiple cis-elements related to disease and stress response consists in GhHsp40 and GhHsp70 promoters. Further expression analysis showed that eight GhHsp40s (Hsp40-2,4,8,11,20,23,53,55) and seven GhHsp70s (Hsp70-2,3,6,8,13,19,22) were up-regulated after infection. In addition, five GhHsp40s (Hsp40-2,8,11,53,55) and four GhHsp70s (Hsp70-3,6,8,13) were up-regulated after salt treatment, six GhHsp40s (Hsp40-4,11,20,23) and three GhHsp70s (Hsp70-2,8,19) were up-regulated after drought treatment, four GhHsp40s (Hsp40-2,11,20,23) and four GhHsp70s (Hsp70-3,6,19,22) were up-regulated after temperature treatment, suggesting these Hsps have possible important function in the process of abiotic stress response. Our results lay a foundation for understanding the function of Hsp40 and Hsp70 in the resistance against and abiotic stress, and elucidating the regulatory mechanism of the protein complex, evolution and molecular mechanism under stress.