Tension can directly suppress Aurora B kinase-triggered release of kinetochore-microtubule attachments

Chromosome segregation requires sister kinetochores to attach microtubules emanating from opposite spindle poles. Proper attachments come under tension and are stabilized, but defective attachments lacking tension are released, giving another chance for correct attachments to form. This error correc...

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Bibliographic Details
Published in:Nature communications 2022-04, Vol.13 (1), p.2152-2152, Article 2152
Main Authors: de Regt, Anna K., Clark, Cordell J., Asbury, Charles L., Biggins, Sue
Format: Article
Language:English
Subjects:
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Accessories
Aurora B protein
Aurora Kinase B - genetics
Chromosome Segregation
Chromosomes
Conformation
Destabilization
Error correction
Humanities and Social Sciences
Kinases
Kinetochores
Microtubules
multidisciplinary
Optical trapping
Science
Science (multidisciplinary)
Spindle Poles
Tension
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Summary:Chromosome segregation requires sister kinetochores to attach microtubules emanating from opposite spindle poles. Proper attachments come under tension and are stabilized, but defective attachments lacking tension are released, giving another chance for correct attachments to form. This error correction process depends on Aurora B kinase, which phosphorylates kinetochores to destabilize their microtubule attachments. However, the mechanism by which Aurora B distinguishes tense versus relaxed kinetochores remains unclear because it is difficult to detect kinase-triggered detachment and to manipulate kinetochore tension in vivo. To address these challenges, we apply an optical trapping-based assay using soluble Aurora B and reconstituted kinetochore-microtubule attachments. Strikingly, the tension on these attachments suppresses their Aurora B-triggered release, suggesting that tension-dependent changes in the conformation of kinetochores can regulate Aurora B activity or its outcome. Our work uncovers the basis for a key mechano-regulatory event that ensures accurate segregation and may inform studies of other mechanically regulated enzymes. Tension stabilizes properly attached microtubules to kinetochores during chromosome segregation, and lack of tension leads to release. Here the authors show that tension directly suppresses Aurora B kinase mediated destabilization of reconstituted kinetochore-microtubule attachments, likely ensuring accurate chromosome segregation.
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-022-29542-8