Exogenous 3,3'-Diindolylmethane Improves Vanadium Stress Tolerance in Brassica napus Seedling Shoots by Modulating Antioxidant Enzyme Activities

• Published in: Biomolecules (Basel, Switzerland) Switzerland), 2021-03, Vol.11 (3), p.436
• Main Authors: Gokul, Arun, Fahiem Carelse, Mogamat, Niekerk, Lee-Ann, Klein, Ashwil, Ludidi, Ndiko, Mendoza-Cozatl, David, Keyster, Marshall
• Format: Article
• Language: English
• Subjects: 3,3′-diindolylmethane; Acids; antioxidant enzymes; Antioxidants; Ascorbic acid; Brassica napus; By products; Cadmium; Cell viability; Chlorophyll; Cultivars; Dry weight; Enzymatic activity; Enzymes; Feeds; Glutathione transferase; Hydrogen peroxide; Hydroxyl radicals; Indoles; L-Ascorbate peroxidase; Lactoylglutathione lyase; Lipid peroxidation; Lipids; Malondialdehyde; Nitrogen; Plant growth; Pyruvaldehyde; Rape plants; Reactive oxygen species; Seed germination; Seedlings; Seeds; Spectroscopy; Superoxide dismutase; Vanadium
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom11030436

3,3'-diindolylmethane (DIM) belongs to a family of indole glucosinolate compounds that have been shown to improve growth through the modulation of reactive oxygen species when applied exogenously. The cultivar AV Garnet was previously identified as a vanadium-sensitive cultivar. Therefore, in this study we investigated whether exogenous DIM could improve the vanadium tolerance of AV Garnet. We performed the following experiments: seed germination assessment, dry weight assessment, cell viability assay, chlorophyll content assay, malondialdehyde (MDA) assay, conjugated diene (CD) content assay, hydrogen peroxide (H O ) content assay, superoxide (O ) content determination, methylglyoxal (MG) content determination, hydroxyl radical ( OH) concentration determination, ascorbate peroxidase (APX) activity assay, superoxide dismutase (SOD) activity assay, glyoxalase I (Gly I) activity assay, glutathione S-transferase (GST) activity assay and inductively coupled plasma optical emission spectroscopy (ICP-OES) analysis for vanadium content determination. Under vanadium stress, exogenous DIM increased the seed germination percentage, shoot dry weight, cell viability and chlorophyll content. Exogenous DIM also led to a decrease in MDA, CD, H O , O , MG and OH, under vanadium stress in the shoots. Furthermore, DIM application led to an increase in the enzymatic activities of APX, SOD, Gly I and GST under vanadium stress. Interestingly, under vanadium stress, DIM treatment did not alter vanadium content in shoots. Our results indicate that exogenous application of DIM can improve seedling shoot growth and biomass under vanadium stress by priming the antioxidant enzymes via reactive oxygen species (ROS) signaling.