Effects of Chestnut Tannin Extract, Vescalagin and Gallic Acid on the Dimethyl Acetals Profile and Microbial Community Composition in Rumen Liquor: An In Vitro Study

The addition of polyphenol extracts in ruminant diets is an effective strategy to modulate rumen microflora. The aim of this in vitro trial was to study the effects of chestnut tannin extract (CHT), vescalagin (VES) and gallic acid (GAL) on dietary fibre degradability and on the dimethyl acetals (DM...

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Published in:Microorganisms (Basel) 2019-07, Vol.7 (7), p.202
Main Authors: Mannelli, Federica, Daghio, Matteo, Alves, Susana P, Bessa, Rui J B, Minieri, Sara, Giovannetti, Luciana, Conte, Giuseppe, Mele, Marcello, Messini, Anna, Rapaccini, Stefano, Viti, Carlo, Buccioni, Arianna
Format: Article
Language:English
Subjects:
Acetals
Acids
chestnut tannin
Community composition
Composition
Degradability
Diet
Dietary fiber
Dimethyl acetals
Environmental impact
Feeds
Fermentation
Gallic acid
Gas chromatography
HTS-technology
Laboratories
Liquor
Microbial activity
Microbiomes
Microflora
Microorganisms
Polyphenols
rRNA 16S
Rumen
rumen microbiota
Synergistic effect
Tannins
vescalagin
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Summary:The addition of polyphenol extracts in ruminant diets is an effective strategy to modulate rumen microflora. The aim of this in vitro trial was to study the effects of chestnut tannin extract (CHT), vescalagin (VES) and gallic acid (GAL) on dietary fibre degradability and on the dimethyl acetals (DMA) profile and microbial community composition of rumen liquor. Four diets (basal diet; basal diet plus CHT; basal diet plus VES; basal diet plus GAL) were fermented for 24 h using ewe rumen liquor. At the end of the fermentation, the microbial communities were characterized by sequencing the 16S rRNA gene. The DMA profile was analyzed by gas chromatography. Chestnut tannin extract did not affect fibre degradability, whereas VES and GAL showed a detrimental effect. The presence of CHT, VES and GAL influenced the concentration of several DMA (i.e., 12:0, 13:0, 14:0, 15:0, 18:0 and 18:1 -11), whereas the composition of the microbial community was marginally affected. The inclusion of CHT led to the enrichment of the genera , , , and . The results of this study support the hypothesis that the activity of CHT is due to the synergistic effect of all components rather than the property of a single component.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms7070202