Analysis of Cardioprotective Effects Using Purified Salvia miltiorrhiza Extract on Isolated Rat Hearts

• Published in: Journal of Pharmacological Sciences 2006, Vol.101(3), pp.245-249
• Main Authors: Piek Ngoh, Chang, Jian Chun, Mao, Shan Hong, Huang, Li, Ning, Zhong Jing, Wang, Todd, On, Wei, Duan, Yi Zhun, Zhu
• Format: Article
• Language: English
• Subjects: Animals; cardioprotective effect; Cardiotonic Agents - pharmacology; Heart - drug effects; In Vitro Techniques; isolated rat heart; Male; Myocardial Contraction; Myocardial Ischemia - physiopathology; myocardial ischemia/reperfusion; Myocardial Reperfusion Injury - prevention & control; Perfusion; Plant Extracts - pharmacology; purified Salvia miltiorrhiza extract; Rats; Rats, Wistar; Salvia miltiorrhiza; Ventricular Function, Left
• ISSN: 1347-8613; 1347-8648
• DOI: 10.1254/jphs.fpj05034x

The purpose of the current study is to evaluate the cardioprotective effects of purified Salvia miltiorrhiza extract (PSME) on myocardial ischemia/reperfusion injury in isolated rat hearts. Hearts were excised and perfused at constant flow (7 – 9 ml min−1) via the aorta. Non-recirculating perfusion with Krebs-Henseleit (KH) solution was maintained at 37°C and continuously gassed with 95% O2 and 5% CO2. KH solution with or without PSME (100 mg per liter solution) was used after 30-min zero-flow ischemia for the PSME and control group, respectively. Left ventricular (LV) developed pressure; its derivatives, diastolic pressure, and so on were continuously recorded via a pressure transducer attached to a polyvinylchloride balloon that was placed in the left ventricle through an incision in the left atrium. PSME treated hearts showed significant postischemic contractile function recovery (developed pressure recovered to 44.2 ± 4.9% versus 17.1 ± 5.7%, P