Surface Modification Strategies for Chrysin-Loaded Iron Oxide Nanoparticles to Boost Their Anti-Tumor Efficacy in Human Colon Carcinoma Cells

• Published in: Journal of functional biomaterials 2024-02, Vol.15 (2), p.43
• Main Authors: Karimova, Aynura, Hajizada, Sabina, Shirinova, Habiba, Nuriyeva, Sevinj, Gahramanli, Lala, Yusuf, Mohammed M, Bellucci, Stefano, Reissfelder, Christoph, Yagublu, Vugar
• Format: Article
• Language: English
• Subjects: Anticancer properties; Apoptosis; Biocompatibility; Cancer; Cancer therapies; Cell viability; Chemotherapy; Chitosan; coating agents; Coatings; Colon; Colon cancer; Colorectal cancer; Crystallites; Crystals; Drug carriers; drug concentration; Drug delivery; Drug therapy; Effectiveness; Efficiency; Ethylenediaminetetraacetic acid; Ferric oxide; Iron compounds; Iron oxides; loading efficiency; magnetite nanoparticles; Nanoparticles; Parameters; Polyethylene glycol; Polymers; Product enhancement; Spectrometry; Spectrum analysis; Systems stability; Toxicity; Tumors
• ISSN: 2079-4983; 2079-4983
• DOI: 10.3390/jfb15020043

Enhancing nanoparticles' anti-cancer capabilities as drug carriers requires the careful adjustment of formulation parameters, including loading efficiency, drug/carrier ratio, and synthesis method. Small adjustments to these parameters can significantly influence the drug-loading efficiency of nanoparticles. Our study explored how chitosan and polyethylene glycol (PEG) coatings affect the structural properties, drug-loading efficiency, and anti-cancer efficacy of Fe O nanoparticles (NPs). The loading efficiency of the NPs was determined using FTIR spectrometry and XRD. The quantity of chrysin incorporated into the coated NPs was examined using UV-Vis spectrometry. The effect of the NPs on cell viability and apoptosis was determined by employing the HCT 116 human colon carcinoma cell line. We showed that a two-fold increase in drug concentration did not impact the loading efficiency of Fe O NPs coated with PEG. However, there was a 33 Å difference in the crystallite sizes obtained from chitosan-coated Fe O NPs and drug concentrations of 1:0.5 and 1:2, resulting in decreased system stability. In conclusion, PEG coating exhibited a higher loading efficiency of Fe O NPs compared to chitosan, resulting in enhanced anti-tumor effects. Furthermore, variations in the loaded amount of chrysin did not impact the crystallinity of PEG-coated NPs, emphasizing the stability and regularity of the system.