The Identification and Validation of Hub Genes Associated with Acute Myocardial Infarction Using Weighted Gene Co-Expression Network Analysis

Acute myocardial infarction (AMI), one of the most severe and fatal cardiovascular diseases, remains the main cause of mortality and morbidity worldwide. The objective of this study is to investigate the potential biomarkers for AMI based on bioinformatics analysis. A total of 2102 differentially ex...

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Bibliographic Details
Published in:Journal of cardiovascular development and disease 2022-01, Vol.9 (1), p.30
Main Authors: Xue, Junqiang, Chen, Lu, Cheng, Hao, Song, Xiaoyue, Shi, Yuekai, Li, Linnan, Xu, Rende, Qin, Qing, Ma, Jianying, Ge, Junbo
Format: Article
Language:English
Subjects:
acute myocardial infarction
bioinformatics
Cardiovascular disease
Gene expression
Genomes
GSEA
Heart attacks
hub genes
Immune system
Ischemia
Medical imaging
Ontology
Proteins
Software
WGCNA
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Summary:Acute myocardial infarction (AMI), one of the most severe and fatal cardiovascular diseases, remains the main cause of mortality and morbidity worldwide. The objective of this study is to investigate the potential biomarkers for AMI based on bioinformatics analysis. A total of 2102 differentially expressed genes (DEGs) were screened out from the data obtained from the gene expression omnibus (GEO) database. Weighted gene co-expression network analysis (WGCNA) explored the co-expression network of DEGs and determined the key module. The brown module was selected as the key one correlated with AMI. Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses demonstrated that genes in the brown module were mainly enriched in 'ribosomal subunit' and 'Ribosome'. Gene Set Enrichment Analysis revealed that 'TNFA_SIGNALING_VIA_NFKB' was remarkably enriched in AMI. Based on the protein-protein interaction network, ribosomal protein L9 (RPL9) and ribosomal protein L26 (RPL26) were identified as the hub genes. Additionally, the polymerase chain reaction (PCR) results indicated that the expression levels of RPL9 and RPL26 were both downregulated in AMI patients compared with controls, in accordance with the bioinformatics analysis. In summary, the identified DEGs, modules, pathways, and hub genes provide clues and shed light on the potential molecular mechanisms of AMI.
ISSN:2308-3425
2308-3425
DOI:10.3390/jcdd9010030