The Heart Protection Effect of Alcalase Potato Protein Hydrolysate Is through IGF1R-PI3K-Akt Compensatory Reactivation in Aging Rats on High Fat Diets

• Published in: International journal of molecular sciences 2015-05, Vol.16 (5), p.10158-10172
• Main Authors: Hu, Wei-Syun, Ting, Wei-Jen, Chiang, Wen-Dee, Pai, Peiying, Yeh, Yu-Lan, Chang, Chung-Ho, Lin, Wan-Teng, Huang, Chih-Yang
• Format: Article
• Language: English
• Subjects: aging; Aging - metabolism; alcalase potato protein hydrolysate; Animals; Anticholesteremic Agents - administration & dosage; Anticholesteremic Agents - pharmacology; Apoptosis; Cardiotonic Agents - administration & dosage; Cardiotonic Agents - pharmacology; Cholesterol - blood; Diet; Diet, High-Fat - adverse effects; Dietary Supplements; Fas-Associated Death Domain Protein - metabolism; Heart; hyperlipidemia; Hyperlipidemias - drug therapy; Hyperlipidemias - etiology; Lipoproteins, LDL - blood; Phosphatidylinositol 3-Kinases - metabolism; Plant Proteins - chemistry; Potatoes; Probucol - pharmacology; Protein Hydrolysates - administration & dosage; Protein Hydrolysates - pharmacology; Proteins; Proto-Oncogene Proteins c-akt - metabolism; Rats; Rats, Sprague-Dawley; Receptor, IGF Type 1 - metabolism; Rodents; Signal Transduction; Solanum tuberosum; Solanum tuberosum - chemistry; Subtilisins - chemistry; Triglycerides - blood
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms160510158

The prevalence of obesity is high in older adults. Alcalase potato protein hydrolysate (APPH), a nutraceutical food, might have greater benefits and be more economical than hypolipidemic drugs. In this study, serum lipid profiles and heart protective effects were evaluated in high fat diet (HFD) induced hyperlipidemia in aging rats treated with APPH (15, 45 and 75 mg/kg/day) and probucol (500 mg/kg/day). APPH treatments reduced serum triacylglycerol (TG), total cholesterol (TC), and low density lipoprotein (LDL) levels to the normal levels expressed in the control group. Additionally, the IGF1R-PI3K-Akt survival pathway was reactivated, and Fas-FADD (Fas-associated death domain) induced apoptosis was inhibited by APPH treatments (15 and 45 mg/kg/day) in HFD aging rat hearts. APPH (75 mg/kg/day) rather than probucol (500 mg/kg/day) treatment could reduce serum lipids without affecting HDL expression. The heart protective effect of APPH in aging rats with hyperlipidemia was through lowering serum lipids and enhancing the activation of the compensatory IGF1R-PI3K-Akt survival pathway.