Arsenic-induced changes in the gene expression of lung epithelial L2 cells: implications in carcinogenesis

Arsenic is a carcinogen that is known to induce cell transformation and tumor formation. Although studies have been performed to examine the modulation of signaling molecules caused by arsenic exposure, the molecular mechanisms by which arsenic causes cancer are still unclear. We hypothesized that a...

Full description

Saved in:
Bibliographic Details
Published in:BMC genomics 2008-03, Vol.9 (1), p.115-115, Article 115
Main Authors: Posey, Tisha, Weng, Tingting, Chen, Zhongming, Chintagari, Narendranath R, Wang, Pengcheng, Jin, Nili, Stricker, Heidi, Liu, Lin
Format: Article
Language:English
Subjects:
Animals
Arsenic
Arsenic - toxicity
Carcinogens - toxicity
Cell Differentiation
Cell Survival
Cell Transformation, Neoplastic - chemically induced
Cell Transformation, Neoplastic - drug effects
Cells, Cultured
Cluster Analysis
Dose-Response Relationship, Drug
Epithelial Cells - cytology
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Gene expression
Gene Expression - drug effects
Genetic aspects
Health aspects
Lung - cytology
Lung - drug effects
Lung - metabolism
Lung cancer
Lung Neoplasms - metabolism
Oligonucleotide Array Sequence Analysis
Rats
Risk factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Arsenic is a carcinogen that is known to induce cell transformation and tumor formation. Although studies have been performed to examine the modulation of signaling molecules caused by arsenic exposure, the molecular mechanisms by which arsenic causes cancer are still unclear. We hypothesized that arsenic alters gene expression leading to carcinogenesis in the lung. In this study, we examined global gene expression in response to 0.75 microM arsenic treatment for 1-7 days in a rat lung epithelial cell line (L2) using an in-house 10 k rat DNA microarray. One hundred thirty one genes were identified using the one-class statistical analysis of microarray (SAM) test. Of them, 33 genes had a fold change of > or = 2 between at least two time points. These genes were then clustered into 5 groups using K-means cluster analysis based on their expression patterns. Seven selected genes, all associated with cancer, were confirmed by real-time PCR. These genes have functions directly or indirectly related to metabolism, glycolysis, cell proliferation and differentiation, and regulation of transcription. Our findings provide important insight for the future studies of arsenic-mediated lung cancer.
ISSN:1471-2164
1471-2164
DOI:10.1186/1471-2164-9-115