Peptide Aggregation Induced Immunogenic Rupture (PAIIR)

Immunogenic cell death (ICD) arises when cells are under stress, and their membranes are damaged. They release damage‐associated molecular patterns (DAMPs) that stimulate and drive the type and magnitude of the immune response. In the presence of an antigen, DAMPs ride the longevity and efficacy of...

Full description

Saved in:
Bibliographic Details
Published in:Advanced science 2022-07, Vol.9 (21), p.e2105868-n/a
Main Authors: Gunay, Gokhan, Hamsici, Seren, Lang, Gillian A., Lang, Mark L., Kovats, Susan, Acar, Handan
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic
Amino acids
Animals
Antibodies
Antibodies, Viral
Antigens
damage‐associated molecular patterns
Design
Hemagglutinin Glycoproteins, Influenza Virus - chemistry
Humans
immunogenic cell death
Immunotherapy
Influenza
influenza vaccination
Influenza Vaccines
Influenza, Human
Mice
Mice, Inbred BALB C
Pathogens
peptide aggregation
Peptides
Proteins
Vaccines
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Immunogenic cell death (ICD) arises when cells are under stress, and their membranes are damaged. They release damage‐associated molecular patterns (DAMPs) that stimulate and drive the type and magnitude of the immune response. In the presence of an antigen, DAMPs ride the longevity and efficacy of antigen‐specific immunity. Yet, no tool can induce the controlled ICD with predictable results. A peptide‐based tool, [II], is designed that aggregates in the cell and causes cell membrane damage, generates ICD and DAMPs release on various cell types, and hence can act as an adjuvant. An influenza vaccine is prepared by combining [II] with influenza hemagglutinin (HA) subunit antigens. The results show that [II] induced significantly higher HA‐specific immunoglobulin G1 (IgG1) and IgG2a antibodies than HA‐only immunized mice, while the peptide itself did not elicit antibodies. This paper demonstrates the first peptide‐aggregation induced immunogenic rupture (PAIIR) approach as a vaccine adjuvant. PAIIR is a promising adjuvant with a high potential to promote universal protection upon influenza HA vaccination. Peptide‐aggregation induced immunogenic rupture on the cell membrane induces the release of damage associated molecular patterns, which can activate the antigen presenting cells and adaptive immune response.
ISSN:2198-3844
2198-3844
DOI:10.1002/advs.202105868