Helicobacter pylori isolation, serology and cagA, cagE and virB11 detection in patients with non-ulcer dyspepsia from Turkey: Correlation with histopathologic findings

Colonization with Helicobacter pylori (HP) may have major clinical consequences and HP virulence factors are associated with more severe gastroduodenal pathologies. In this study, prevalence of HP in patients with Non-Ulcer Dyspepsia (NUD) was determined by rapid urease test and culture and correlat...

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Published in:Central European journal of medicine 2008-03, Vol.3 (1), p.41-46
Main Authors: Ilga, Ugur, Ozyurt, Mustafa, Yildirim, Sukru, Ergunay, Koray, Ardic, Nurittin, Demirturk, Levent, Haznedaroglu, Tuncer
Format: Article
Language:English
Subjects:
Biomedicine
CagA
Helicobacter pylori
Internal Medicine
Maternal and Child Health
Medicine
Medicine & Public Health
Non-ulcer dyspepsia
Pathogenesis
Reproductive Medicine
Research Article
Serology
Surgery
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Summary:Colonization with Helicobacter pylori (HP) may have major clinical consequences and HP virulence factors are associated with more severe gastroduodenal pathologies. In this study, prevalence of HP in patients with Non-Ulcer Dyspepsia (NUD) was determined by rapid urease test and culture and correlations of histopathologic changes with bacterial virulence factors and serologic profiles were investigated. Gastric biopsies from sixty-nine patients admitted to Haydarpasa Training Hospital Department of Gastroenterology were evaluated for rapid urease, HP isolation and examined histopathologically. PCR was employed for HP confirmation and detection of HP cag A, cag E and vir B11 genes. For each patient, IgG and IgA antibodies and anti- cag A antibodies were also determined by ELISA tests. HP was isolated and confirmed by PCR in 74% (51/69) of the patients. Anti-HP IgG and IgA were detected in 96% (49/51) and 53% (27/51), respectively. Anti-cagA were present in 51% (26/51). cag A, cag E and vir B11 were positive in 56.8% (29/51), 60.7% (31/51) and 58.8% (30/51) of the patients, respectively. Statistically significant correlation was observed between cag A PCR and inflammation/activity scores. Detection of cag A by molecular assays can be an alternative test that can be employed for individual patient assessment.
ISSN:1895-1058
2391-5463
1644-3640
2391-5463
DOI:10.2478/s11536-007-0062-y