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Helicobacter pylori isolation, serology and cagA, cagE and virB11 detection in patients with non-ulcer dyspepsia from Turkey: Correlation with histopathologic findings
Colonization with Helicobacter pylori (HP) may have major clinical consequences and HP virulence factors are associated with more severe gastroduodenal pathologies. In this study, prevalence of HP in patients with Non-Ulcer Dyspepsia (NUD) was determined by rapid urease test and culture and correlat...
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| Published in: | Central European journal of medicine 2008-03, Vol.3 (1), p.41-46 |
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| container_end_page | 46 |
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| container_start_page | 41 |
| container_title | Central European journal of medicine |
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| creator | Ilga, Ugur Ozyurt, Mustafa Yildirim, Sukru Ergunay, Koray Ardic, Nurittin Demirturk, Levent Haznedaroglu, Tuncer |
| description | Colonization with
Helicobacter pylori
(HP) may have major clinical consequences and HP virulence factors are associated with more severe gastroduodenal pathologies. In this study, prevalence of HP in patients with Non-Ulcer Dyspepsia (NUD) was determined by rapid urease test and culture and correlations of histopathologic changes with bacterial virulence factors and serologic profiles were investigated. Gastric biopsies from sixty-nine patients admitted to Haydarpasa Training Hospital Department of Gastroenterology were evaluated for rapid urease, HP isolation and examined histopathologically. PCR was employed for HP confirmation and detection of HP
cag
A,
cag
E and
vir
B11 genes. For each patient, IgG and IgA antibodies and anti-
cag
A antibodies were also determined by ELISA tests. HP was isolated and confirmed by PCR in 74% (51/69) of the patients. Anti-HP IgG and IgA were detected in 96% (49/51) and 53% (27/51), respectively. Anti-cagA were present in 51% (26/51).
cag
A,
cag
E and
vir
B11 were positive in 56.8% (29/51), 60.7% (31/51) and 58.8% (30/51) of the patients, respectively. Statistically significant correlation was observed between
cag
A PCR and inflammation/activity scores. Detection of
cag
A by molecular assays can be an alternative test that can be employed for individual patient assessment. |
| doi_str_mv | 10.2478/s11536-007-0062-y |
| format | article |
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Helicobacter pylori
(HP) may have major clinical consequences and HP virulence factors are associated with more severe gastroduodenal pathologies. In this study, prevalence of HP in patients with Non-Ulcer Dyspepsia (NUD) was determined by rapid urease test and culture and correlations of histopathologic changes with bacterial virulence factors and serologic profiles were investigated. Gastric biopsies from sixty-nine patients admitted to Haydarpasa Training Hospital Department of Gastroenterology were evaluated for rapid urease, HP isolation and examined histopathologically. PCR was employed for HP confirmation and detection of HP
cag
A,
cag
E and
vir
B11 genes. For each patient, IgG and IgA antibodies and anti-
cag
A antibodies were also determined by ELISA tests. HP was isolated and confirmed by PCR in 74% (51/69) of the patients. Anti-HP IgG and IgA were detected in 96% (49/51) and 53% (27/51), respectively. Anti-cagA were present in 51% (26/51).
cag
A,
cag
E and
vir
B11 were positive in 56.8% (29/51), 60.7% (31/51) and 58.8% (30/51) of the patients, respectively. Statistically significant correlation was observed between
cag
A PCR and inflammation/activity scores. Detection of
cag
A by molecular assays can be an alternative test that can be employed for individual patient assessment.</description><identifier>ISSN: 1895-1058</identifier><identifier>ISSN: 2391-5463</identifier><identifier>EISSN: 1644-3640</identifier><identifier>EISSN: 2391-5463</identifier><identifier>DOI: 10.2478/s11536-007-0062-y</identifier><language>eng</language><publisher>Warsaw: Versita</publisher><subject>Biomedicine ; CagA ; Helicobacter pylori ; Internal Medicine ; Maternal and Child Health ; Medicine ; Medicine & Public Health ; Non-ulcer dyspepsia ; Pathogenesis ; Reproductive Medicine ; Research Article ; Serology ; Surgery</subject><ispartof>Central European journal of medicine, 2008-03, Vol.3 (1), p.41-46</ispartof><rights>Versita Warsaw and Springer-Verlag Berlin Heidelberg 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c430t-b850e842efb03c6f967f43abc128b0226b728afbb0032319c4358a31e6481fba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.2478/s11536-007-0062-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.2478/s11536-007-0062-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41418,42487,51318,67158,68942</link.rule.ids></links><search><creatorcontrib>Ilga, Ugur</creatorcontrib><creatorcontrib>Ozyurt, Mustafa</creatorcontrib><creatorcontrib>Yildirim, Sukru</creatorcontrib><creatorcontrib>Ergunay, Koray</creatorcontrib><creatorcontrib>Ardic, Nurittin</creatorcontrib><creatorcontrib>Demirturk, Levent</creatorcontrib><creatorcontrib>Haznedaroglu, Tuncer</creatorcontrib><title>Helicobacter pylori isolation, serology and cagA, cagE and virB11 detection in patients with non-ulcer dyspepsia from Turkey: Correlation with histopathologic findings</title><title>Central European journal of medicine</title><addtitle>cent.eur.j.med</addtitle><description>Colonization with
Helicobacter pylori
(HP) may have major clinical consequences and HP virulence factors are associated with more severe gastroduodenal pathologies. In this study, prevalence of HP in patients with Non-Ulcer Dyspepsia (NUD) was determined by rapid urease test and culture and correlations of histopathologic changes with bacterial virulence factors and serologic profiles were investigated. Gastric biopsies from sixty-nine patients admitted to Haydarpasa Training Hospital Department of Gastroenterology were evaluated for rapid urease, HP isolation and examined histopathologically. PCR was employed for HP confirmation and detection of HP
cag
A,
cag
E and
vir
B11 genes. For each patient, IgG and IgA antibodies and anti-
cag
A antibodies were also determined by ELISA tests. HP was isolated and confirmed by PCR in 74% (51/69) of the patients. Anti-HP IgG and IgA were detected in 96% (49/51) and 53% (27/51), respectively. Anti-cagA were present in 51% (26/51).
cag
A,
cag
E and
vir
B11 were positive in 56.8% (29/51), 60.7% (31/51) and 58.8% (30/51) of the patients, respectively. Statistically significant correlation was observed between
cag
A PCR and inflammation/activity scores. Detection of
cag
A by molecular assays can be an alternative test that can be employed for individual patient assessment.</description><subject>Biomedicine</subject><subject>CagA</subject><subject>Helicobacter pylori</subject><subject>Internal Medicine</subject><subject>Maternal and Child Health</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Non-ulcer dyspepsia</subject><subject>Pathogenesis</subject><subject>Reproductive Medicine</subject><subject>Research Article</subject><subject>Serology</subject><subject>Surgery</subject><issn>1895-1058</issn><issn>2391-5463</issn><issn>1644-3640</issn><issn>2391-5463</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNqNkc-O1SAYxRujiePoA7gjrqfKx79SExfjzehMMombcU2AQi_XTqnQOukT-ZrSqdGViQvgg5zfOSSnql4DfktYI99lAE5FjXFTliD1-qQ6A8FYTQXDT8ssW14D5vJ59SLnU9HgppVn1c9rNwQbjbazS2hah5gCCjkOeg5xvEDZpTjEfkV67JDV_eXFtl89Xn-E9BEAdW52dlOjMKKpcG6cM3oI8xGNcayXwRbnbs2Tm3LQyKd4j-6W9M2t79EhpuT2rJ04hjzHYnLcUoNFPoxdGPv8snrm9ZDdq9_nefX109Xd4bq-_fL55nB5W1tG8VwbybGTjDhvMLXCt6LxjGpjgUiDCRGmIVJ7YzCmhEJbKC41BSeYBG80Pa9udt8u6pOaUrjXaVVRB_X4EFOvdJqDHZxqABtvW2w4LRmtaD0GLoU3wKwwAMXrze41pfh9cXlWp7iksXxfESpaDi3nRQS7yKaYc3L-TyhgtVWr9mpVqVZt1aq1MB925kEPpbbO9WlZy_DX_98ssO1jZOdzyRr7_wN_AdrWvXE</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Ilga, Ugur</creator><creator>Ozyurt, Mustafa</creator><creator>Yildirim, Sukru</creator><creator>Ergunay, Koray</creator><creator>Ardic, Nurittin</creator><creator>Demirturk, Levent</creator><creator>Haznedaroglu, Tuncer</creator><general>Versita</general><general>Walter de Gruyter GmbH</general><general>De Gruyter</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>DOA</scope></search><sort><creationdate>20080301</creationdate><title>Helicobacter pylori isolation, serology and cagA, cagE and virB11 detection in patients with non-ulcer dyspepsia from Turkey: Correlation with histopathologic findings</title><author>Ilga, Ugur ; Ozyurt, Mustafa ; Yildirim, Sukru ; Ergunay, Koray ; Ardic, Nurittin ; Demirturk, Levent ; Haznedaroglu, Tuncer</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-b850e842efb03c6f967f43abc128b0226b728afbb0032319c4358a31e6481fba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Biomedicine</topic><topic>CagA</topic><topic>Helicobacter pylori</topic><topic>Internal Medicine</topic><topic>Maternal and Child Health</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Non-ulcer dyspepsia</topic><topic>Pathogenesis</topic><topic>Reproductive Medicine</topic><topic>Research Article</topic><topic>Serology</topic><topic>Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ilga, Ugur</creatorcontrib><creatorcontrib>Ozyurt, Mustafa</creatorcontrib><creatorcontrib>Yildirim, Sukru</creatorcontrib><creatorcontrib>Ergunay, Koray</creatorcontrib><creatorcontrib>Ardic, Nurittin</creatorcontrib><creatorcontrib>Demirturk, Levent</creatorcontrib><creatorcontrib>Haznedaroglu, Tuncer</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection (ProQuest Medical & Health Databases)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Central European journal of medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ilga, Ugur</au><au>Ozyurt, Mustafa</au><au>Yildirim, Sukru</au><au>Ergunay, Koray</au><au>Ardic, Nurittin</au><au>Demirturk, Levent</au><au>Haznedaroglu, Tuncer</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Helicobacter pylori isolation, serology and cagA, cagE and virB11 detection in patients with non-ulcer dyspepsia from Turkey: Correlation with histopathologic findings</atitle><jtitle>Central European journal of medicine</jtitle><stitle>cent.eur.j.med</stitle><date>2008-03-01</date><risdate>2008</risdate><volume>3</volume><issue>1</issue><spage>41</spage><epage>46</epage><pages>41-46</pages><issn>1895-1058</issn><issn>2391-5463</issn><eissn>1644-3640</eissn><eissn>2391-5463</eissn><abstract>Colonization with
Helicobacter pylori
(HP) may have major clinical consequences and HP virulence factors are associated with more severe gastroduodenal pathologies. In this study, prevalence of HP in patients with Non-Ulcer Dyspepsia (NUD) was determined by rapid urease test and culture and correlations of histopathologic changes with bacterial virulence factors and serologic profiles were investigated. Gastric biopsies from sixty-nine patients admitted to Haydarpasa Training Hospital Department of Gastroenterology were evaluated for rapid urease, HP isolation and examined histopathologically. PCR was employed for HP confirmation and detection of HP
cag
A,
cag
E and
vir
B11 genes. For each patient, IgG and IgA antibodies and anti-
cag
A antibodies were also determined by ELISA tests. HP was isolated and confirmed by PCR in 74% (51/69) of the patients. Anti-HP IgG and IgA were detected in 96% (49/51) and 53% (27/51), respectively. Anti-cagA were present in 51% (26/51).
cag
A,
cag
E and
vir
B11 were positive in 56.8% (29/51), 60.7% (31/51) and 58.8% (30/51) of the patients, respectively. Statistically significant correlation was observed between
cag
A PCR and inflammation/activity scores. Detection of
cag
A by molecular assays can be an alternative test that can be employed for individual patient assessment.</abstract><cop>Warsaw</cop><pub>Versita</pub><doi>10.2478/s11536-007-0062-y</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1895-1058 |
| ispartof | Central European journal of medicine, 2008-03, Vol.3 (1), p.41-46 |
| issn | 1895-1058 2391-5463 1644-3640 2391-5463 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_710bfc90b53f43969f01586fb14c6b11 |
| source | Sciendo (De Gruyter) Open Access Journals; Springer Nature - Connect here FIRST to enable access |
| subjects | Biomedicine CagA Helicobacter pylori Internal Medicine Maternal and Child Health Medicine Medicine & Public Health Non-ulcer dyspepsia Pathogenesis Reproductive Medicine Research Article Serology Surgery |
| title | Helicobacter pylori isolation, serology and cagA, cagE and virB11 detection in patients with non-ulcer dyspepsia from Turkey: Correlation with histopathologic findings |
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