A Novel Methodology for Detecting Variations in Cell Surface Antigens Using Cell-Tearing by Optical Tweezers

The quantitative analysis of cell surface antigens has attracted increasing attention due to the antigenic variation recognition that can facilitate early diagnoses. This paper presents a novel methodology based on the optical “cell-tearing” and the especially proposed “dilution regulations” to dete...

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Bibliographic Details
Published in:Biosensors (Basel) 2022-08, Vol.12 (8), p.656
Main Authors: Lin, Chih-Lang, Wang, Shyang-Guang, Tien, Meng-Tsung, Chiang, Chung-Han, Lee, Yi-Chieh, Baldeck, Patrice L, Shin, Chow-Shing
Format: Article
Language:English
Subjects:
agglutination test
Analysis
Antibodies
antigenic variation
Antigens
Binding
Biological markers
Biophysics
Cell surface
Cell surface antigens
cell-tearing
Dilution
Erythrocytes
Experiments
Flow cytometry
Lasers
Methodology
optical tweezers
Physics
Quantitative analysis
Surface antigens
Tearing
Testing
Variation
whole-cell-based analysis
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Summary:The quantitative analysis of cell surface antigens has attracted increasing attention due to the antigenic variation recognition that can facilitate early diagnoses. This paper presents a novel methodology based on the optical “cell-tearing” and the especially proposed “dilution regulations” to detect variations in cell surface antigens. The cell attaches to the corresponding antibody-coated slide surface. Then, the cell-binding firmness between a single cell and the functionalized surface is assayed by optically tearing using gradually reduced laser powers incorporated with serial antibody dilutions. Groups B and B3 of red blood cells (RBCs) were selected as the experiment subject. The results indicate that a higher dilution called for lower power to tear off the cell binding. According to the proposed relative-quantitative analysis theory, antigenic variation can be intuitively estimated by comparing the maximum allowable dilution folds. The estimation result shows good consistency with the finding in the literature. This study suggests a novel methodology for examining the variation in cell surface antigens, expected to be widely capable with potential sensor applications not only in biochemistry and biophysics, but also in the micro-/nano- engineering field.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios12080656