Transcriptome analysis of some genes of Staphylococcus aureus exposed to alkylated, nano- and native chitosans

•Nanoparticles and N-alkyl chitosan were prepared by method of Cross-linking and Schiff base formation respectively.•The formation of modified forms was confirmed by evaluating the physicochemical properties.•Chitosan and its modified forms are known as an antimicrobial agent against Staphylococcus...

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Bibliographic Details
Published in:Carbohydrate polymer technologies and applications 2023-12, Vol.6, p.100351, Article 100351
Main Authors: Mirbagheri, Vasighe Sadat, Alishahi, Alireza, Petroudi, Seyyed Hamid Reza Hashemi, Ojagh, Seyed Mahdi
Format: Article
Language:English
Subjects:
Antibacterial
Cell death
Membrane permeability
Modified chitosan
Staphylococcus aureus
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Summary:•Nanoparticles and N-alkyl chitosan were prepared by method of Cross-linking and Schiff base formation respectively.•The formation of modified forms was confirmed by evaluating the physicochemical properties.•Chitosan and its modified forms are known as an antimicrobial agent against Staphylococcus aureus.•Chitosan and its modified forms because cell wall stress and induction of vraS, vraR, lytM, lrgA, and lrgB genes. Chitosan, including native, N- alkyl (AlkCs), and nanoparticles chitosan (CsNPs), were prepared and characterized. Average size and hydrodynamic diameter of nanoparticles were determined at 126 nm and 240 nm with a polydispersity index (PDI) of 0.154 and zeta potential of 30 mV, respectively. Crystallinity index for Cs, AlkCs, and CsNPs was calculated as 57.85, 30.24, and 24.85, respectively. Minimum concentration of growth inhibition (125.78 µg/ml) and lethality (100 µg/ml) was observed for CsNPs (P 
ISSN:2666-8939
2666-8939
DOI:10.1016/j.carpta.2023.100351