Neutrophils in Extravascular Body Fluids: Cytological-Energy Analysis Enables Rapid, Reliable and Inexpensive Detection of Purulent Inflammation and Tissue Damage

• Published in: Life (Basel, Switzerland) Switzerland), 2022-01, Vol.12 (2), p.160
• Main Authors: Kelbich, Petr, Vachata, Petr, Maly, Vilem, Novotny, Tomas, Spicka, Jan, Matuchova, Inka, Radovnicky, Tomas, Stanek, Ivan, Kubalik, Jan, Karpjuk, Ondrej, Smisko, Frantisek, Hanuljakova, Eva, Krejsek, Jan
• Format: Article
• Language: English
• Subjects: Abdomen; Aspartate aminotransferase; Body fluids; Cerebrospinal fluid; coefficient of energy balance; Damage; Energy; Enzymes; Glucose; Immune response; Inflammation; Laboratories; Leukocytes (neutrophilic); Metabolism; Neutrophils; Phagocytes; pleural effusion; purulent inflammation; Reactive oxygen species; Statistical analysis
• ISSN: 2075-1729; 2075-1729
• DOI: 10.3390/life12020160

The simultaneous cytological and metabolic investigation of various extravascular body fluids (EBFs) provides clinically relevant information about the type and intensity of the immune response in particular organ systems. The oxidative burst of professional phagocytes with the concomitant production of reactive oxygen species consumes a large amount of oxygen and is the cause of switch to the development of anaerobic metabolism. We assessed the relationships between percentages of neutrophils, aerobic and anaerobic metabolism, and tissue damage via the determination of aspartate aminotransferase catalytic activities (AST) in cerebrospinal fluid (CSF), pleural effusions (PE), abdominal effusions (AE), and synovial fluids (SF). 83.0% aerobic and 1.3% strongly anaerobic cases with median of AST = 13.8 IU/L in CSF; 68.0% aerobic and 9.0% strongly anaerobic cases with median of AST = 20.4 IU/L in PE; 77.5% aerobic and 10.5% strongly anaerobic cases with median of AST = 18.0 IU/L in AE; 64.1% aerobic and 7.7% strongly anaerobic cases with median of AST = 13.8 IU/L in SF. 4.2% aerobic and 73.7% strongly anaerobic cases with median of AST = 19.2 IU/L in CSF; 7.4% aerobic and 77.3% strongly anaerobic cases with median of AST = 145.2 IU/L in PE; 11.8% aerobic and 73.7% strongly anaerobic cases with median of AST = 61.8 IU/L in AE; 25.5% aerobic and 38.2% strongly anaerobic cases with median of AST = 37.2 IU/L in SF. The significant presence of neutrophils, concomitant strong anaerobic metabolism, and elevated AST in various EBFs are reliable signs of damaging purulent inflammation.