Production of Polyhydroxyalkanoates (PHAs) by Vibrio alginolyticus Strains Isolated from Salt Fields

Vibrio alginolyticus is a halophilic organism usually found in marine environments. It has attracted attention as an opportunistic pathogen of aquatic animals and humans, but there are very few reports on polyhydroxyalkanoate (PHA) production using V. alginolyticus as the host. In this study, two V....

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2021-10, Vol.26 (20), p.6283
Main Authors: Li, Hong-Fei, Wang, Meng-Ru, Tian, Lin-Yue, Li, Zheng-Jun
Format: Article
Language:English
Subjects:
Antibiotics
Aquatic animals
Bacteria
Biodegradable materials
Biodegradation
Biodiesel fuels
Biofuels
Carbon
Carbon sources
Cell growth
Drug resistance
E coli
Fermentation
Glycerol
Marine environment
Microorganisms
Opportunist infection
Optimization
Organic acids
PHB
PHBV
Plastics
poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
Poly-3-hydroxybutyrate
Polyhydroxyalkanoates
Polyhydroxyalkanoic acid
Polyhydroxybutyrate
Polymerization
Propionic acid
Salt
Sodium chloride
Strains (organisms)
Sugar
Vibrio alginolyticus
Water analysis
Water sampling
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Summary:Vibrio alginolyticus is a halophilic organism usually found in marine environments. It has attracted attention as an opportunistic pathogen of aquatic animals and humans, but there are very few reports on polyhydroxyalkanoate (PHA) production using V. alginolyticus as the host. In this study, two V. alginolyticus strains, LHF01 and LHF02, isolated from water samples collected from salt fields were found to produce poly(3-hydroxybutyrate) (PHB) from a variety of sugars and organic acids. Glycerol was the best carbon source and yielded the highest PHB titer in both strains. Further optimization of the NaCl concentration and culture temperature improved the PHB titer from 1.87 to 5.08 g/L in V. alginolyticus LHF01. In addition, the use of propionate as a secondary carbon source resulted in the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). V. alginolyticus LHF01 may be a promising host for PHA production using cheap waste glycerol from biodiesel refining.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26206283