Transcriptome Analysis Reveals That Abeliophyllum distichum Nakai Extract Inhibits RANKL-Mediated Osteoclastogenensis Mainly through Suppressing Nfatc1 Expression

Abeliophyllum distichum Nakai is known as a monotypic genus endemic to South Korea. Currently, several pharmacological studies have revealed that A. distichum extract exhibits diverse biological functions, including anti-cancer, anti-diabetic, anti-hypertensive, and anti-inflammatory activities. In...

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Published in:Biology (Basel, Switzerland) Switzerland), 2020-08, Vol.9 (8), p.212
Main Authors: Lee, Kyubin, Jang, You-Jee, Lee, Hyerim, Kim, Eunbin, Kim, Yeojin, Yoo, Tong-Kewn, Hyun, Tae Kyung, Park, Jae-Il, Yi, Sun-Ju, Kim, Kyunghwan
Format: Article
Language:English
Subjects:
Abeliophyllum distichum Nakai
Antibodies
Antihypertensives
Bone loss
Bone marrow
Care and treatment
Cell growth
Diabetes mellitus
Extracellular signal-regulated kinase
Fractures
Gene expression
Genetic aspects
Glucocorticoids
Health aspects
Inflammation
Kinases
Materia medica, Vegetable
Medicine, Botanic
Medicine, Herbal
NF-κB protein
NFATc1
osteoclast
Osteoclastogenesis
Osteoclasts (Biology)
Osteoporosis
Ovariectomy
Phosphatase
Phosphorylation
Plant extracts
Post-menopause
TRANCE protein
Transcription factors
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Summary:Abeliophyllum distichum Nakai is known as a monotypic genus endemic to South Korea. Currently, several pharmacological studies have revealed that A. distichum extract exhibits diverse biological functions, including anti-cancer, anti-diabetic, anti-hypertensive, and anti-inflammatory activities. In this study, we present the anti-osteoporotic activity of A. distichum extract by inhibiting osteoclast formation. First, we show that the methanolic extract of the leaves of A. distichum, but not extracts of the branches or fruits, significantly inhibits receptor activator of the NF-κB ligand (RANKL)-induced osteoclast differentiation. Second, our transcriptome analysis revealed that the leaf extract (LE) blocks sets of RANKL-mediated osteoclast-related genes. Third, the LE attenuates the phosphorylation of extracellular signal-related kinase. Finally, treatment with the LE effectively prevents postmenopausal bone loss in ovariectomized mice and glucocorticoid-induced osteoporosis in zebrafish. Our findings show that the extract of A. distichum efficiently suppressed osteoclastogenesis by regulating osteoclast-related genes, thus offering a novel therapeutic strategy for osteoporosis.
ISSN:2079-7737
2079-7737
DOI:10.3390/biology9080212