The Role of NFATC4 Gene in Human Cutaneous Squamous Cell Carcinoma

Nuclear factor of activated T-cells, cytoplasmic 4 (NFATC4) has been implicated in keratinocyte development and several types of cancer. A well-defined role for NFATC4 in cutaneous squamous cell carcinoma (CSCC) has not yet been established. In this study, NFATC4 gene function in CSCC development wa...

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Bibliographic Details
Published in:Indian journal of dermatology 2023-03, Vol.68 (2), p.156-160
Main Authors: Li, Fengjuan, Chen, Hongquan, Lu, Xiaoou
Format: Article
Language:English
Subjects:
Apoptosis
Basic Research
calcineurin
Cancer
carcinogenesis
Cell growth
cutaneous squamous cell carcinoma
Ethylenediaminetetraacetic acid
Genes
Genetic aspects
Genetic transcription
nfatc4
Pathogenesis
RNA
Skin cancer
Squamous cell carcinoma
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Summary:Nuclear factor of activated T-cells, cytoplasmic 4 (NFATC4) has been implicated in keratinocyte development and several types of cancer. A well-defined role for NFATC4 in cutaneous squamous cell carcinoma (CSCC) has not yet been established. In this study, NFATC4 gene function in CSCC development was examined. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to measure the mRNA expression of NFATC4 in CSCC tissues and controls. A431 and Colo16 cell proliferation, invasion, and apoptosis were measured by CCK-8 assay, transwell invasion, and flow cytometry, respectively, after an NFATC4 expression lentivirus infection. Animal models were applied to validate the function of the NFATC4 gene. (1) CSCC tissues showed a significant decrease in NFATC4 expression compared to controls. (2) Overexpression of NFATc4 suppresses A431 and Colo16 cell proliferation and invasion but promotes cell apoptosis. (3) Mouse models overexpressing NFATC4 showed reduced tumourigenesis. It was suggested that NFATC4 might be a tumour suppressor gene in CSCC.
ISSN:0019-5154
1998-3611
DOI:10.4103/ijd.ijd_837_22