Production and Structural Characterization of Lactobacillus helveticus Derived Biosurfactant

A probiotic strain of lactobacilli was isolated from traditional soft Churpi cheese of Yak milk and found positive for biosurfactant production. Lactobacilli reduced the surface tension of phosphate buffer saline (PBS) from 72.0 to 39.5 mNm−1 pH 7.2 and its critical micelle concentration (CMC) was f...

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Bibliographic Details
Published in:TheScientificWorld 2014, Vol.2014 (2014), p.1-9
Main Authors: Bansal, Anshul, Chauhan, Nikhil, Singh Saharan, Baljeet, Sharma, Deepansh, Procha, Suresh
Format: Article
Language:English
Subjects:
Animals
Bacteria
Biomass
Brassica - drug effects
Carbohydrates
Cell Death - drug effects
Cell Line
Chemical properties
Chemical synthesis
Cytotoxicity
Fermentation
Food
Gas Chromatography-Mass Spectrometry
Glycolipids - metabolism
Lactobacillus
Lactobacillus helveticus
Lactobacillus helveticus - chemistry
Lactose
Lactose - analysis
Magnetic Resonance Spectroscopy
Methods
Mice
Pharmaceuticals
Production processes
Proteins
Surface active agents
Surface tension
Surface Tension - drug effects
Surface-Active Agents - chemistry
Surface-Active Agents - metabolism
Surface-Active Agents - toxicity
Surfactants
Thermogravimetry
Toxicity
Triticum - drug effects
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Summary:A probiotic strain of lactobacilli was isolated from traditional soft Churpi cheese of Yak milk and found positive for biosurfactant production. Lactobacilli reduced the surface tension of phosphate buffer saline (PBS) from 72.0 to 39.5 mNm−1 pH 7.2 and its critical micelle concentration (CMC) was found to be 2.5 mg mL−1. Low cost production of Lactobacilli derived biosurfactant was carried out at lab scale fermenter which yields 0.8 mg mL−1 biosurfactant. The biosurfactant was found least phytotoxic and cytotoxic as compared to the rhamnolipid and sodium dodecyl sulphate (SDS) at different concentration. Structural attributes of biosurfactant were determined by FTIR, NMR (1H and 13C), UPLC-MS, and fatty acid analysis by GCMS which confirmed the presence of glycolipid type of biosurfactant closely similar to xylolipids. Biosurfactant is mainly constituted by lipid and sugar fractions. The present study outcomes provide valuable information on structural characterization of the biosurfactant produced by L. helveticus MRTL91. These findings are encouraging for the application of Lactobacilli derived biosurfactant as nontoxic surface active agents in the emerging field of biomedical applications.
ISSN:2356-6140
1537-744X
1537-744X
DOI:10.1155/2014/493548