Detection of Unamplified E. coli O157 DNA Extracted from Large Food Samples Using a Gold Nanoparticle Colorimetric Biosensor

Rapid detection of foodborne pathogens such as O157 is essential in reducing the prevalence of foodborne illness and subsequent complications. Due to their unique colorimetric properties, gold nanoparticles (GNPs) can be applied in biosensor development for affordability and accessibility. In this w...

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Bibliographic Details
Published in:Biosensors (Basel) 2022-04, Vol.12 (5), p.274
Main Authors: Dester, Emma, Kao, Kaily, Alocilja, Evangelyn C
Format: Article
Language:English
Subjects:
biosensing
Biosensing Techniques - methods
Biosensors
Colorimetry
Complications
Deoxyribonucleic acid
DNA
E coli
Escherichia coli O157
Flour
Food
Food Microbiology
food safety
Foodborne diseases
foodborne illness
Foodborne pathogens
Genetic testing
Gold
Laboratories
Metal Nanoparticles
Nanoparticles
oligonucleotide probe
Pathogens
Salmonella
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Summary:Rapid detection of foodborne pathogens such as O157 is essential in reducing the prevalence of foodborne illness and subsequent complications. Due to their unique colorimetric properties, gold nanoparticles (GNPs) can be applied in biosensor development for affordability and accessibility. In this work, a GNP biosensor was designed for visual differentiation between target ( O157:H7) and non-target DNA samples. Results of DNA extracted from pure cultures indicate high specificity and sensitivity to as little as 2.5 ng/µL O157 DNA. Further, the biosensor successfully identified DNA extracted from flour contaminated with O157, with no false positives for flour contaminated with non-target bacteria. After genomic extraction, this assay can be performed in as little as 30 min. In addition, food sample testing was successful at detecting approximately 10 CFU/mL of O157 magnetically extracted from flour after only a 4 h incubation step. As a proof of concept, these results demonstrate the capabilities of this GNP biosensor for low-cost and rapid foodborne pathogen detection.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios12050274