Pre-Formulation Studies: Physicochemical Characteristics and In Vitro Release Kinetics of Insulin from Selected Hydrogels

Insulin loaded to the polymer network of hydrogels may affect the speed and the quality of wound healing in diabetic patients. The aim of our research was to develop a formulation of insulin that could be applied to the skin. We chose hydrogels commonly used for pharmaceutical compounding, which can...

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Bibliographic Details
Published in:Pharmaceutics 2021-08, Vol.13 (8), p.1215
Main Authors: Ostróżka-Cieślik, Aneta, Maciążek-Jurczyk, Małgorzata, Pożycka, Jadwiga, Dolińska, Barbara
Format: Article
Language:English
Subjects:
Amino acids
Cell growth
Cellulose
Diabetes
Epidermal growth factor
Fibroblasts
Foot diseases
Glucose
Glycerol
hydrogel
Hydrogels
in vitro drug release study
insulin
Insulin resistance
Kinases
Leg ulcers
Metabolism
Nitric oxide
Peptides
Proteins
rheology
Signal transduction
Skin
spectroscopic methods
texture analysis
Wound healing
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Summary:Insulin loaded to the polymer network of hydrogels may affect the speed and the quality of wound healing in diabetic patients. The aim of our research was to develop a formulation of insulin that could be applied to the skin. We chose hydrogels commonly used for pharmaceutical compounding, which can provide a form of therapy available to every patient. We prepared different gel formulations using Carbopol® UltrezTM 10, Carbopol® UltrezTM 30, methyl cellulose, and glycerin ointment. The hormone concentration was 1 mg/g of the hydrogel. We assessed the influence of model hydrogels on the pharmaceutical availability of insulin in vitro, and we examined the rheological and the texture parameters of the prepared formulations. Based on spectroscopic methods, we evaluated the influence of model hydrogels on secondary and tertiary structures of insulin. The analysis of rheograms showed that hydrogels are typical of shear-thinning non-Newtonian thixotropic fluids. Insulin release from the formulations occurs in a prolonged manner, providing a longer duration of action of the hormone. The stability of insulin in hydrogels was confirmed. The presence of model hydrogel carriers affects the secondary and the tertiary structures of insulin. The obtained results indicate that hydrogels are promising carriers in the treatment of diabetic foot ulcers. The most effective treatment can be achieved with a methyl cellulose-based insulin preparation.
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics13081215