Early Neurodegeneration after Hypoxia-Ischemia in Neonatal Rat Is Necrosis while Delayed Neuronal Death Is Apoptosis

We used silver staining to demonstrate neuronal cell body, axonal, and terminal degeneration in brains from p7 rat pups recovered for 0, 1.5, 3, 6, 24, 48, 72 h, and 6 days following hypoxia-ischemia. We found that initial injury is evident in ipsilateral forebrain by 3 h following hypoxia-ischemia,...

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Bibliographic Details
Published in:Neurobiology of disease 2001-04, Vol.8 (2), p.207-219
Main Authors: Northington, Frances J., Ferriero, Donna M., Graham, Ernest M., Traystman, Richard J., Martin, Lee J.
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn - anatomy & histology
Animals, Newborn - injuries
Animals, Newborn - metabolism
Apoptosis - physiology
Asphyxia Neonatorum - metabolism
Asphyxia Neonatorum - pathology
Asphyxia Neonatorum - physiopathology
Brain - pathology
Brain - physiopathology
Brain - ultrastructure
Fluorescent Dyes - pharmacokinetics
Humans
Hypoxia-Ischemia, Brain - metabolism
Hypoxia-Ischemia, Brain - pathology
Hypoxia-Ischemia, Brain - physiopathology
Infant, Newborn
Microscopy, Electron
Necrosis
Nerve Degeneration - etiology
Nerve Degeneration - pathology
Nerve Degeneration - physiopathology
Neural Pathways - pathology
Neural Pathways - physiopathology
Neurons - metabolism
Neurons - pathology
Neurons - ultrastructure
Rats
Silver Staining
Stilbamidines
Time Factors
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Summary:We used silver staining to demonstrate neuronal cell body, axonal, and terminal degeneration in brains from p7 rat pups recovered for 0, 1.5, 3, 6, 24, 48, 72 h, and 6 days following hypoxia-ischemia. We found that initial injury is evident in ipsilateral forebrain by 3 h following hypoxia-ischemia, while injury in ventral basal thalamus develops at 24 h. A secondary phase of injury occurs at 48 h in ipsilateral cortex, but not until 6 days in basal ganglia. Initial injury in striatum and cortex is necrosis, but in thalamus the neurodegeneration is primarily apoptosis. Degeneration also occurs in bilateral white matter tracts, and in synaptic terminal fields associated with apoptosis in regions remote from the primary injury. These results show that hypoxia-ischemia in the developing brain causes both early and delayed neurodegeneration in specific systems in which the morphology of neuronal death is determined by time, region, and potentially by patterns of neuronal connectivity.
ISSN:0969-9961
1095-953X
DOI:10.1006/nbdi.2000.0371