Neutrophil lncRNA ZNF100-6:2 is a potential diagnostic marker for active pulmonary tuberculosis

Active pulmonary tuberculosis (PTB) poses challenges in rapid diagnosis within complex clinical conditions. Given the close association between neutrophils and tuberculosis, we explored differentially expressed long non-coding RNAs (lncRNAs) in neutrophils as potential molecular markers for diagnosi...

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Bibliographic Details
Published in:European journal of medical research 2024-03, Vol.29 (1), p.162-8, Article 162
Main Authors: Huang, Shuying, Kang, Xiuhua, Zeng, Zhenguo, Zhang, Qilong, Huang, Zikun, Luo, Kaihang, Yao, Qinqin, Chen, Bing, Qing, Cheng
Format: Article
Language:English
Subjects:
Biomarkers
DNA microarrays
Ethics
Ethylenediaminetetraacetic acid
Experiments
Gene expression
Genes
HIV
Human immunodeficiency virus
Humans
Hybridization
Infections
Infectious diseases
Instrument industry
lncRNA ZNF100-6:2
Long non-coding RNAs
Neutrophils
Pulmonary tuberculosis
RNA, Long Noncoding - genetics
Software
Tuberculosis
Tuberculosis, Pulmonary
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Summary:Active pulmonary tuberculosis (PTB) poses challenges in rapid diagnosis within complex clinical conditions. Given the close association between neutrophils and tuberculosis, we explored differentially expressed long non-coding RNAs (lncRNAs) in neutrophils as potential molecular markers for diagnosing active PTB. We employed a gene microarray to screen for lncRNA alterations in neutrophil samples from three patients with active PTB and three healthy controls. The results revealed differential expression of 1457 lncRNAs between the two groups, with 916 lncRNAs upregulated and 541 lncRNAs down-regulated in tuberculosis patients. Subsequent validation tests demonstrated down-regulation of lncRNA ZNF100-6:2 in patients with active PTB, which was restored following anti-tuberculosis treatment. Our findings further indicated a high diagnostic potential for lncRNA ZNF100-6:2, as evidenced by an area under the receiver operating characteristic (ROC) curve of 0.9796 (95% confidence interval: 0.9479 to 1.000; P 
ISSN:2047-783X
0949-2321
2047-783X
DOI:10.1186/s40001-024-01755-1